Recombinant hnRNP G Antibody - Primary antibody, specific to hnRNP G, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human hnRNP G Antibody; WB, IHC, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab108427
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab108427-10μl
10μl
In stock
$69.90
Ab108427-50μl
50μl
In stock
$189.90
Ab108427-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab108427-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human hnRNP G Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant hnRNP G Antibody - Primary antibody, specific to hnRNP G, Rabbit IgG
SynonymsGlycoprotein p43 | Heterogeneous nuclear ribonucleoprotein G | hnRNP G | Glycoprotein p43 antibody | Heterogeneous nuclear ribonucleoprotein G antibody | hnRNP G antibody | HNRNPG antibody | HNRPG antibody | RBMX antibody | RBMXP1 antibody | RBMXRT antibo
Specifications & PurityExactAb™, Validated, Recombinant, 0.4 mg/mL
Host speciesRabbit
SpecificityhnRNP G
ImmunogenA synthetic peptide derived from human hnRNP G (AA 341-354)
Positive ControlWB: K562, HeLa, Jurkat, A431, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: 293T cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human hnRNP G Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Relevance:
Heterogeneous nuclear ribonuclearproteins (hnRNPs) are nucleic acid binding proteins and have critical roles in DNA repair, telomere regulation, and transcriptional gene regulation. hnRNP G has tumor suppressive activity and this RNA binding protein which may be involved in pre-mRNA splicing.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.4 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant hnRNP G Antibody (Ab108427) - Western Blot
All lanes: Recombinant hnRNP G Antibody (Ab108427) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 18.8 s

Recombinant hnRNP G Antibody (Ab108427) - IF/ICC
Immunocytochemistry analysis of hnRNP G (green) in 293T using Recombinant hnRNP G Antibody (Ab108427), and cells were counterstained with DAPI (blue).

Recombinant hnRNP G Antibody (Ab108427) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant hnRNP G Antibody (Ab108427). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

RBMX Tbio RNA-binding motif protein, X chromosome (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506059Certificate of AnalysisMay 23, 2024 Ab108427
ZJ24F0506060Certificate of AnalysisMay 23, 2024 Ab108427

Related Documents

References

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2. Soulard, M M and 9 more authors..  (1993)  hnRNP G: sequence and characterization of a glycosylated RNA-binding protein..  Nucleic acids research,    (11):   [PMID:7692398]
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4. Lingenfelter, P A PA and 6 more authors..  (2001)  Expression and conservation of processed copies of the RBMX gene..  Mammalian genome : official journal of the International Mammalian Genome Society,      [PMID:11420617]
5. Jurica, Melissa S MS, Licklider, Lawrence J LJ, Gygi, Steven R SR, Grigorieff, Nikolaus N and Moore, Melissa J MJ..  (2002)  Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis..  RNA (New York, N.Y.),      [PMID:11991638]
6. Hofmann, Yvonne Y and Wirth, Brunhilde B..  (2002)  hnRNP-G promotes exon 7 inclusion of survival motor neuron (SMN) via direct interaction with Htra2-beta1..  Human molecular genetics,    (15):   [PMID:12165565]
7. Nasim, M Talat MT, Chernova, Tatyana K TK, Chowdhury, Hasnin M HM, Yue, Bai-Gong BG and Eperon, Ian C IC..  (2003)  HnRNP G and Tra2beta: opposite effects on splicing matched by antagonism in RNA binding..  Human molecular genetics,    (1):   [PMID:12761049]
8. Ross, Mark T MT and 281 more authors..  (2005)  The DNA sequence of the human X chromosome..  Nature,    (17):   [PMID:15772651]
9. Shin, Ki-Hyuk KH, Kang, Mo K MK, Kim, Reuben H RH, Christensen, Russell R and Park, No-Hee NH..  (2006)  Heterogeneous nuclear ribonucleoprotein G shows tumor suppressive effect against oral squamous cell carcinoma cells..  Clinical cancer research : an official journal of the American Association for Cancer Research,    (15):   [PMID:16707624]
10. Ulke-Lemée, Annegret A and 7 more authors..  (2007)  The nuclear PP1 interacting protein ZAP3 (ZAP) is a putative nucleoside kinase that complexes with SAM68, CIA, NF110/45, and HNRNP-G..  Biochimica et biophysica acta,      [PMID:17890166]
11. Adamik, Barbara B and 5 more authors..  (2008)  An association between RBMX, a heterogeneous nuclear ribonucleoprotein, and ARTS-1 regulates extracellular TNFR1 release..  Biochemical and biophysical research communications,    (4):   [PMID:18445477]
12. Shin, Ki-Hyuk KH, Kim, Reuben H RH, Kim, Roy H RH, Kang, Mo K MK and Park, No-Hee NH..  (2008)  hnRNP G elicits tumor-suppressive activity in part by upregulating the expression of Txnip..  Biochemical and biophysical research communications,    (8):   [PMID:18541147]
13. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:18669648]
14. Heinrich, Bettina B and 12 more authors..  (2009)  Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA..  The Journal of biological chemistry,    (22):   [PMID:19282290]
15. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
16. Kanhoush, Rasha R and 5 more authors..  ()  Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts..  Nucleus (Austin, Tex.),      [PMID:21327109]
17. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
18. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
19. Shashi, V V and 10 more authors..  (2015)  The RBMX gene as a candidate for the Shashi X-linked intellectual disability syndrome..  Clinical genetics,      [PMID:25256757]
20. Lauranzano, Eliana E and 12 more authors..  (2015)  Peptidylprolyl isomerase A governs TARDBP function and assembly in heterogeneous nuclear ribonucleoprotein complexes..  Brain : a journal of neurology,      [PMID:25678563]
21. Xiao, Zhenyu Z and 5 more authors..  (2015)  System-wide Analysis of SUMOylation Dynamics in Response to Replication Stress Reveals Novel Small Ubiquitin-like Modified Target Proteins and Acceptor Lysines Relevant for Genome Stability..  Molecular & cellular proteomics : MCP,      [PMID:25755297]
22. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O..  (2015)  SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage..  Cell reports,    (17):   [PMID:25772364]
23. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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