Recombinant; Rabbit anti Human Id1 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab109300-10μl | 10μl | In stock | $89.90 | |
| Ab109300-50μl | 50μl | In stock | $269.90 | |
| Ab109300-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $419.90 | |
| Ab109300-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human Id1 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant Id1 Antibody - Primary antibody, specific to Id1, Rabbit IgG |
|---|---|
| Synonyms | bHLHb24 antibody; Class B basic helix-loop-helix protein 24 antibody; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody; DNA binding protein inhibitor ID 1 antibody; DNA binding protein inhibitor ID1 antibody; |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.2 mg/mL |
| Host species | Rabbit |
| Specificity | Id1 |
| Immunogen | A synthetic peptide derived from human Id1 (AA 1-63). |
| Positive Control | WB: A549, A431, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human kidney. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Id1 Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Id1 Antibody (Ab109300) - Western Blot
All lanes: Recombinant Id1 Antibody (Ab109300) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 26 kDa
Exposure time: 31.5 s
Recombinant Id1 Antibody (Ab109300) - IHC
Immunohistochemistry analysis of paraffin-embedded Human kidney using Recombinant Id1 Antibody (Ab109300). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval
Recombinant Id1 Antibody (Ab109300) - IF/ICC
Immunofluorescence analysis of ID1 in HeLa using Recombinant Id1 Antibody (Ab109300).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303889 | Certificate of Analysis | Apr 02, 2024 | Ab109300 |
| ZJ24F0303888 | Certificate of Analysis | Apr 02, 2024 | Ab109300 |
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| 2. Betzi S, Restouin A, Opi S, Arold ST, Parrot I, Guerlesquin F, Morelli X, Collette Y.. (2007) Protein protein interaction inhibition (2P2I) combining high throughput and virtual screening: Application to the HIV-1 Nef protein.. Proc Natl Acad Sci U S A, 104 (49): (19256-19261). [PMID:18042718] |
| 3. Zhu, W W and 8 more authors.. (1995) Id gene expression during development and molecular cloning of the human Id-1 gene.. Brain research. Molecular brain research, [PMID:7637581] |
| 4. Deed, R W RW, Jasiok, M M and Norton, J D JD.. (1994) Nucleotide sequence of the cDNA encoding human helix-loop-helix Id-1 protein: identification of functionally conserved residues common to Id proteins.. Biochimica et biophysica acta, (13): [PMID:8086456] |
| 5. Hara, E E and 6 more authors.. (1994) Id-related genes encoding helix-loop-helix proteins are required for G1 progression and are repressed in senescent human fibroblasts.. The Journal of biological chemistry, (21): [PMID:8294468] |
| 6. Nehlin, J O JO, Hara, E E, Kuo, W L WL, Collins, C C and Campisi, J J.. (1997) Genomic organization, sequence, and chromosomal localization of the human helix-loop-helix Id1 gene.. Biochemical and biophysical research communications, (24): [PMID:9070860] |
| 7. Ward, Sarah M SM, Fernando, Shanik J SJ, Hou, Tim Y TY and Duffield, Giles E GE.. (2010) The transcriptional repressor ID2 can interact with the canonical clock components CLOCK and BMAL1 and mediate inhibitory effects on mPer1 expression.. The Journal of biological chemistry, (10): [PMID:20861012] |
