Recombinant; Rabbit anti Human Ihh Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab109665-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab109665-50μl | 50μl | In stock | $199.90 | |
| Ab109665-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab109665-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human Ihh Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant Ihh Antibody - Primary antibody, specific to Ihh, Rabbit IgG |
|---|---|
| Synonyms | BDA1 antibody; HHG-2 antibody; HHG2 antibody; IHH antibody; IHH_HUMAN antibody; Indian Hedgehog antibody; Indian hedgehog homolog antibody; Indian hedgehog protein antibody; Indian hedgehog protein C-product antibody; Indian hedgehog protein N-product ant |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | Ihh |
| Immunogen | A synthetic peptide derived from human Ihh (AA 28-42) |
| Positive Control | WB: Hep G2, LNCaP, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Mouse colon tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Ihh Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Ihh Antibody (Ab109665) - Western Blot
All lanes: Recombinant Ihh Antibody (Ab109665) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 41 kDa
Exposure time: 10.7 s
Recombinant Ihh Antibody (Ab109665) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant Ihh Antibody (Ab109665). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202164 | Certificate of Analysis | Dec 20, 2023 | Ab109665 |
| ZJ23F1202163 | Certificate of Analysis | Dec 20, 2023 | Ab109665 |
| 1. Marigo, V V and 9 more authors.. (1995) Cloning, expression, and chromosomal location of SHH and IHH: two human homologues of the Drosophila segment polarity gene hedgehog.. Genomics, (1): [PMID:7590746] |
| 2. Chang, D T DT and 8 more authors.. (1994) Products, genetic linkage and limb patterning activity of a murine hedgehog gene.. Development (Cambridge, England), [PMID:7720571] |
| 3. Pepinsky, R B RB and 12 more authors.. (1998) Identification of a palmitic acid-modified form of human Sonic hedgehog.. The Journal of biological chemistry, (29): [PMID:9593755] |
| 4. Gao, B B and 9 more authors.. (2001) Mutations in IHH, encoding Indian hedgehog, cause brachydactyly type A-1.. Nature genetics, [PMID:11455389] |
| 5. McCready, M Elizabeth ME and 8 more authors.. (2002) A novel mutation in the IHH gene causes brachydactyly type A1: a 95-year-old mystery resolved.. Human genetics, [PMID:12384778] |
| 6. Hellemans, Jan J and 6 more authors.. (2003) Homozygous mutations in IHH cause acrocapitofemoral dysplasia, an autosomal recessive disorder with cone-shaped epiphyses in hands and hips.. American journal of human genetics, [PMID:12632327] |
| 7. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 8. Kavran, Jennifer M JM, Ward, Matthew D MD, Oladosu, Oyindamola O OO, Mulepati, Sabin S and Leahy, Daniel J DJ.. (2010) All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner.. The Journal of biological chemistry, (6): [PMID:20519495] |
| 9. Queiroz, Karla C S KC and 10 more authors.. (2010) Human plasma very low density lipoprotein carries Indian hedgehog.. Journal of proteome research, (5): [PMID:20839884] |
| 10. Ma, Gang G and 18 more authors.. (2011) Indian hedgehog mutations causing brachydactyly type A1 impair Hedgehog signal transduction at multiple levels.. Cell research, [PMID:21537345] |
| 11. Pettigrew, Christopher A CA, Asp, Eva E and Emerson, Charles P CP.. (2014) A new role for Hedgehogs in juxtacrine signaling.. Mechanisms of development, [PMID:24342078] |
