Recombinant Integrin alpha 2 Antibody - Primary antibody, specific to ITGA2, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab110755
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab110755-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab110755-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$189.90
Ab110755-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$279.90
Ab110755-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

Recombinant; Rabbit anti Human Integrin alpha 2 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant Integrin alpha 2 Antibody - Primary antibody, specific to ITGA2, Rabbit IgG
SynonymsBR antibody | CD 49b antibody | CD49 antigen like family member B antibody | CD49 antigen-like family member B antibody | CD49b antibody | CD49b antigen antibody | Collagen receptor antibody | DX5 antibody | Glycoprotein Ia deficiency included antibody |
Specifications & PurityExactAb™, Validated, Recombinant, 1.6 mg/mL
Host speciesRabbit
SpecificityITGA2
ImmunogenA synthetic peptide derived from human Integrin alpha 2 (AA 1100-1181).
Positive ControlWB: A431, HEK293 and HUVEC cell lysates. IHC: Mouse kidney tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Integrin alpha 2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function:
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. 

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration1.6 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Integrin alpha 2 Antibody (Ab110755) - Western Blot
All lanes: Recombinant Integrin alpha 2 Antibody (Ab110755) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 150 kDa
Observed band size: 147 kDa
Exposure time: 5.6 s

Recombinant Integrin alpha 2 Antibody (Ab110755) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue using Recombinant Integrin alpha 2 Antibody (Ab110755). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

ITGA2 Tbio Integrin alpha-2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0901117Certificate of AnalysisSep 24, 2023 Ab110755
ZJ23F0901116Certificate of AnalysisSep 24, 2023 Ab110755

Related Documents

References

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2. Nissinen L, Koivunen J, Käpylä J, Salmela M, Nieminen J, Jokinen J, Sipilä K, Pihlavisto M, Pentikäinen OT, Marjamäki A et al..  (2012)  Novel α2β1 integrin inhibitors reveal that integrin binding to collagen under shear stress conditions does not require receptor preactivation..  J Biol Chem,  287  (53): (44694-702).  [PMID:23132859] [10.1021/op500134e]
3. San Sebastián E, Zimmerman T, Zubia A, Vara Y, Martin E, Sirockin F, Dejaegere A, Stote RH, Lopez X, Pantoja-Uceda D, Valcárcel M, Mendoza L, Vidal-Vanaclocha F, Cossío FP, Blanco FJ..  (2013)  Design, synthesis, and functional evaluation of leukocyte function associated antigen-1 antagonists in early and late stages of cancer development..  J Med Chem,  56  (3): (735-747).  [PMID:23339734] [10.1021/jm3016848]
4. Bhunia SS, Misra A, Khan IA, Gaur S, Jain M, Singh S, Saxena A, Hohlfield T, Dikshit M, Saxena AK..  (2017)  Novel Glycoprotein VI Antagonists as Antithrombotics: Synthesis, Biological Evaluation, and Molecular Modeling Studies on 2,3-Disubstituted Tetrahydropyrido(3,4-b)indoles..  J Med Chem,  60  (1): (322-337).  [PMID:27996269] [10.1021/acs.jmedchem.6b01360]
5. Takada, Y Y and Hemler, M E ME..  (1989)  The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain..  The Journal of cell biology,      [PMID:2545729]
6. Santoso, S S and 5 more authors..  (1993)  The human platelet alloantigens Br(a) and Brb are associated with a single amino acid polymorphism on glycoprotein Ia (integrin subunit alpha 2)..  The Journal of clinical investigation,      [PMID:7901236]
7. Zutter, M M MM, Santoro, S A SA, Painter, A S AS, Tsung, Y L YL and Gafford, A A..  (1994)  The human alpha 2 integrin gene promoter. Identification of positive and negative regulatory elements important for cell-type and developmentally restricted gene expression..  The Journal of biological chemistry,    (7):   [PMID:8276836]
8. Emsley, J J, King, S L SL, Bergelson, J M JM and Liddington, R C RC..  (1997)  Crystal structure of the I domain from integrin alpha2beta1..  The Journal of biological chemistry,    (7):   [PMID:9353312]
9. Kroll, H H, Gardemann, A A, Fechter, A A, Haberbosch, W W and Santoso, S S..  (2000)  The impact of the glycoprotein Ia collagen receptor subunit A1648G gene polymorphism on coronary artery disease and acute myocardial infarction..  Thrombosis and haemostasis,      [PMID:10744142]
10. Schmutz, Jeremy J and 75 more authors..  (2004)  The DNA sequence and comparative analysis of human chromosome 5..  Nature,    (16):   [PMID:15372022]
11. Lewandrowski, Urs U, Moebius, Jan J, Walter, Ulrich U and Sickmann, Albert A..  (2006)  Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach..  Molecular & cellular proteomics : MCP,      [PMID:16263699]
12. Pellinen, Teijo T and 5 more authors..  (2006)  Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins..  The Journal of cell biology,    (5):   [PMID:16754960]
13. Zahedi, René P RP and 8 more authors..  (2008)  Phosphoproteome of resting human platelets..  Journal of proteome research,      [PMID:18088087]
14. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:18669648]
15. Pellinen, Teijo T and 12 more authors..  (2008)  Integrin trafficking regulated by Rab21 is necessary for cytokinesis..  Developmental cell,      [PMID:18804435]
16. Wollscheid, Bernd B and 7 more authors..  (2009)  Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins..  Nature biotechnology,      [PMID:19349973]
17. Bertrand, G G and 9 more authors..  (2013)  HPA-5 typing discrepancy reveals an Ile503Leu substitution in platelet GPIa (α2 integrin)..  Vox sanguinis,      [PMID:23368983]
18. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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