A synthetic peptide derived from human Integrin alpha 5 (AA 1037-1049).
Positive Control
WB: A549, U937, HUVEC, HeLa, K562, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Integrin alpha 5 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Integrin alpha 5 Antibody (Ab110802) - Western Blot All lanes: Recombinant Integrin alpha 5 Antibody (Ab110802) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 115 kDa Observed band size: 17, 139 kDa Exposure time: 15.0 s
Recombinant Integrin alpha 5 Antibody (Ab110802) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Integrin alpha 5 Antibody (Ab110802). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.