A synthetic peptide derived from human Integrin alpha 6 (AA 25-53).
Positive Control
WB: A431 and HT-29 cell lysates. IHC: Human uterus.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Integrin alpha 6 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Integrin alpha 6 Antibody (Ab110811) - Western Blot All lanes: Recombinant Integrin alpha 6 Antibody (Ab110811) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 125 kDa Observed band size: 124 kDa Exposure time: 16.1 s
Recombinant Integrin alpha 6 Antibody (Ab110811) - IHC Immunohistochemistry analysis of paraffin-embedded Human uterus using Recombinant Integrin alpha 6 Antibody (Ab110811). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.