ExactAb™, Validated, Recombinant, High performance, 0.25 mg/mL
Host species
Rabbit
Specificity
IRF4
Immunogen
A synthetic peptide derived from human MUM1 (AA 144-450).
Positive Control
WB: Ramos cell lysate.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Product Description
Rabbit anti Human IRF4 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 – 1/10000 Protein Function Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.25 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant IRF4 Antibody (Ab116484) - Western Blot All lanes: Recombinant IRF4 Antibody (Ab116484) at 1/10000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 52 kDa Observed band size: 50 kDa Exposure time: 76.6 s
1.Grossman, A A and 9 more authors.. (1996) Cloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25.. Genomics, (15):[PMID:8921401]
2.Iida, S S and 7 more authors.. (1997) Deregulation of MUM1/IRF4 by chromosomal translocation in multiple myeloma.. Nature genetics, [PMID:9326949]
3.Rao, S S, Matsumura, A A, Yoon, J J and Simon, M C MC.. (1999) SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1.. The Journal of biological chemistry, (16):[PMID:10196196]
4.Gupta, Sanjay S and 6 more authors.. (2003) Molecular cloning of IBP, a SWAP-70 homologous GEF, which is highly expressed in the immune system.. Human immunology, [PMID:12651066]
5.Han, Jiali J and 16 more authors.. (2008) A genome-wide association study identifies novel alleles associated with hair color and skin pigmentation.. PLoS genetics, (16):[PMID:18483556]
6.Praetorius, Christian and 32 more authors.. (2013) A polymorphism in IRF4 affects human pigmentation through a tyrosinase-dependent MITF/TFAP2A pathway.. Cell, (21):[PMID:24267888]
Solution Calculators
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.