Recombinant ISG15 Antibody - Primary antibody, specific to ISG15, Rabbit IgG

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab111307
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab111307-10μl
10μl
In stock
$79.90
Ab111307-50μl
50μl
In stock
$239.90
Ab111307-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$349.90
Ab111307-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,899.90

Recombinant; Rabbit anti Human ISG15 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant ISG15 Antibody - Primary antibody, specific to ISG15, Rabbit IgG
SynonymsG1P2 antibody | hUCRP antibody | IFI 15 antibody | IFI15 antibody | Interferon alpha inducible protein antibody | Interferon induced protein 15 antibody | Interferon induced protein IFI-15K antibody | Interferon stimulated protein 15 kDa antibody | Interf
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityISG15
ImmunogenA synthetic peptide derived from human ISG15 (AA 141-154)
Positive ControlWB: HeLa, Hep G2 and MCF7 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human ISG15 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function
Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant ISG15 Antibody (Ab111307) - Western Blot
All lanes: Recombinant ISG15 Antibody (Ab111307) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa
Exposure time: 200.0 s

Recombinant ISG15 Antibody (Ab111307) - Western Blot
All lanes: Recombinant ISG15 Antibody (Ab111307) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa
Exposure time: 10.0 s

Associated Targets(Human)

ISG15 Tbio Ubiquitin-like protein ISG15 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506073Certificate of AnalysisMay 24, 2024 Ab111307
ZJ24F0506074Certificate of AnalysisMay 24, 2024 Ab111307

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