Basic Description

Product Name	Recombinant ISG15 Antibody - Primary antibody, specific to ISG15, Rabbit IgG
Synonyms	G1P2 antibody \| hUCRP antibody \| IFI 15 antibody \| IFI15 antibody \| Interferon alpha inducible protein antibody \| Interferon induced protein 15 antibody \| Interferon induced protein IFI-15K antibody \| Interferon stimulated protein 15 kDa antibody \| Interf
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species	Rabbit
Specificity	ISG15
Immunogen	A synthetic peptide derived from human ISG15 (AA 141-154)
Positive Control	WB: HeLa, Hep G2 and MCF7 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human ISG15 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.2 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant ISG15 Antibody (Ab111307) - Western Blot
All lanes: Recombinant ISG15 Antibody (Ab111307) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa
Exposure time: 200.0 s

Recombinant ISG15 Antibody (Ab111307) - Western Blot
All lanes: Recombinant ISG15 Antibody (Ab111307) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa
Exposure time: 10.0 s

Associated Targets(Human)

ISG15 Tbio Ubiquitin-like protein ISG15 (0 Activities)

Discover Target: ISG15

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0506073	Certificate of Analysis	May 24, 2024	Ab111307
ZJ24F0506074	Certificate of Analysis	May 24, 2024	Ab111307

FAQs and Articles

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Guidelines of Primary Antibodies for Western Blotting

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Protocol of Western Blotting For High Molecular Weights

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SKU	Size	Availability	Price
Ab111307-10μl	10μl	In stock	$79.90
Ab111307-50μl	50μl	In stock	$239.90
Ab111307-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$349.90
Ab111307-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,899.90

Recombinant ISG15 Antibody - Primary antibody, specific to ISG15, Rabbit IgG