A synthetic peptide derived from human JNK1 (AA 325-343)
Positive Control
WB: C6, U937, HeLa, MCF7 and Jurkat cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human JNK1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 - 1/1000 ICC/IF: 1/50-1/200 Protein Function Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. Post-translational Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
AI Insight
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant JNK1 Antibody (Ab111518) - Western Blot All lanes: Recombinant JNK1 Antibody (Ab111518) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 42 kDa Exposure time: 60.0 s
Associated Targets(Human)
MAPK8TchemMitogen-activated protein kinase 8 (0 Activities)
ZJ24F0102421