| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab111758-10μl | 10μl | In stock | $59.90 | |
| Ab111758-50μl | 50μl | In stock | $179.90 | |
| Ab111758-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab111758-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human KAT2B/PCAF Antibody; WB; Unconjugated
| Product Name | Recombinant KAT2B/PCAF Antibody - Primary antibody, specific to KAT2B, Rabbit IgG |
|---|---|
| Synonyms | 2.3.1.57 | EC 2.3.1.48 | Histone acetylase PCAF | Histone acetyltransferase PCAF | Lysine acetyltransferase 2B | P/CAF | P300/CBP-associated factor | Spermidine acetyltransferase KAT2B |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.22 mg/mL |
| Host species | Rabbit |
| Specificity | KAT2B |
| Immunogen | A synthetic peptide derived from human KAT2B/PCAF (AA 154-190) |
| Positive Control | WB: HeLa, HEK293, A431, Hep G2 and LNCaP cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human KAT2B/PCAF Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.22 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant KAT2B/PCAF Antibody (Ab111758) - Western Blot
All lanes: Recombinant KAT2B/PCAF Antibody (Ab111758) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 93 kDa
Observed band size: 86 kDa
Exposure time: 9.3 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303368 | Certificate of Analysis | Mar 19, 2024 | Ab111758 |
| ZJ24F0303369 | Certificate of Analysis | Mar 19, 2024 | Ab111758 |
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| 38. Dhalluin, C C and 5 more authors.. (1999) Structure and ligand of a histone acetyltransferase bromodomain.. Nature, (3): [PMID:10365964] |
| 39. Harrod, R R and 6 more authors.. (2000) p300 and p300/cAMP-responsive element-binding protein associated factor interact with human T-cell lymphotropic virus type-1 Tax in a multi-histone acetyltransferase/activator-enhancer complex.. The Journal of biological chemistry, (21): [PMID:10766811] |
| 40. Chakraborty, S S, Senyuk, V V, Sitailo, S S, Chi, Y Y and Nucifora, G G.. (2001) Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) results in reversible acetylation of EVI1 and in co-localization in nuclear speckles.. The Journal of biological chemistry, (30): [PMID:11568182] |
| 41. Mujtaba, Shiraz S and 7 more authors.. (2002) Structural basis of lysine-acetylated HIV-1 Tat recognition by PCAF bromodomain.. Molecular cell, [PMID:11931765] |
| 42. Brès, Vanessa V and 8 more authors.. (2002) Differential acetylation of Tat coordinates its interaction with the co-activators cyclin T1 and PCAF.. The EMBO journal, (16): [PMID:12486002] |
| 43. Curtis, Anne M AM and 7 more authors.. (2004) Histone acetyltransferase-dependent chromatin remodeling and the vascular clock.. The Journal of biological chemistry, (20): [PMID:14645221] |
| 44. Gangisetty, Omkaram O, Lauffart, Brenda B, Sondarva, Gautam V GV, Chelsea, Diane M DM and Still, Ivan H IH.. (2004) The transforming acidic coiled coil proteins interact with nuclear histone acetyltransferases.. Oncogene, (1): [PMID:14767476] |
| 45. Zhao, Quan Q, Cumming, Helen H, Cerruti, Loretta L, Cunningham, John M JM and Jane, Stephen M SM.. (2004) Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1.. The Journal of biological chemistry, (1): [PMID:15273251] |
| 46. Huq, M D Mostaqul MD, Gupta, Pawan P, Tsai, Nien-Pei NP and Wei, Li-Na LN.. (2006) Modulation of testicular receptor 4 activity by mitogen-activated protein kinase-mediated phosphorylation.. Molecular & cellular proteomics : MCP, [PMID:16887930] |
| 47. Shin, Sook S and Janknecht, Ralf R.. (2007) Concerted activation of the Mdm2 promoter by p72 RNA helicase and the coactivators p300 and P/CAF.. Journal of cellular biochemistry, (1): [PMID:17226766] |
| 48. Wiper-Bergeron, Nadine N, Salem, Houssein Abdou HA, Tomlinson, Julianna J JJ, Wu, Dongmei D and Haché, Robert J G RJ.. (2007) Glucocorticoid-stimulated preadipocyte differentiation is mediated through acetylation of C/EBPbeta by GCN5.. Proceedings of the National Academy of Sciences of the United States of America, (20): [PMID:17301242] |
| 49. Gururaj, Anupama E AE, Peng, Shaohua S, Vadlamudi, Ratna K RK and Kumar, Rakesh R.. (2007) Estrogen induces expression of BCAS3, a novel estrogen receptor-alpha coactivator, through proline-, glutamic acid-, and leucine-rich protein-1 (PELP1).. Molecular endocrinology (Baltimore, Md.), [PMID:17505058] |
| 50. Zhu, Ping P and 8 more authors.. (2007) A histone H2A deubiquitinase complex coordinating histone acetylation and H1 dissociation in transcriptional regulation.. Molecular cell, (17): [PMID:17707232] |
| 51. Pickard, Adam A, Wong, Ping-Pui PP and McCance, Dennis J DJ.. (2010) Acetylation of Rb by PCAF is required for nuclear localization and keratinocyte differentiation.. Journal of cell science, (1): [PMID:20940255] |
| 52. Xia, Peng P and 10 more authors.. (2012) EB1 acetylation by P300/CBP-associated factor (PCAF) ensures accurate kinetochore-microtubule interactions in mitosis.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:23001180] |
| 53. Lin, Ruiting R and 7 more authors.. (2013) Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth.. Molecular cell, (22): [PMID:23932781] |
| 54. Gong, Fade F and 11 more authors.. (2015) Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination.. Genes & development, (15): [PMID:25593309] |
| 55. Chen, Sifan S and 6 more authors.. (2016) SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing.. Nature communications, (12): [PMID:26867678] |
| 56. Fournier, Marjorie M and 11 more authors.. (2016) KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification.. Nature communications, (31): [PMID:27796307] |
| 57. Rainger, Joe J and 15 more authors.. (2017) A recurrent de novo mutation in ACTG1 causes isolated ocular coloboma.. Human mutation, [PMID:28493397] |
| 58. Ghosh, Tushar K TK and 7 more authors.. (2018) Acetylation of TBX5 by KAT2B and KAT2A regulates heart and limb development.. Journal of molecular and cellular cardiology, [PMID:29174768] |
