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Recombinant KAT2B/PCAF Antibody - Primary antibody, specific to KAT2B, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human KAT2B/PCAF Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab111758
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab111758-10μl
10μl
In stock
$59.90
Ab111758-50μl
50μl
In stock
$179.90
Ab111758-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab111758-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90
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Recombinant; Rabbit anti Human KAT2B/PCAF Antibody; WB; Unconjugated

View related series
Click to view target information: KAT2B
Accession#:Q92831 Gene ID:8850 KAT2B PCAF

Basic Description

Product NameRecombinant KAT2B/PCAF Antibody - Primary antibody, specific to KAT2B, Rabbit IgG
SynonymsCAF; P/CAF; PCAF; GCN5; GCN5L; GCN5L1; KAT2B; Pcaf; CAF antibody; CREBBP associated factor antibody; GCN5 antibody; GCN5L antibody; GCN5L1 antibody; Histone acetylase PCAF antibody; Histone acetyltransferase KAT2B antibody; Histone acetyltransferase PCAF
Specifications & PurityExactAb™, Validated, Recombinant, 0.22 mg/mL
Host speciesRabbit
SpecificityKAT2B
ImmunogenA synthetic peptide derived from human KAT2B/PCAF (AA 154-190)
Positive ControlWB: HeLa, HEK293, A431, Hep G2 and LNCaP cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human KAT2B/PCAF Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function:
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.22 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant KAT2B/PCAF Antibody (Ab111758) - Western Blot
All lanes: Recombinant KAT2B/PCAF Antibody (Ab111758) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 93 kDa
Observed band size: 86 kDa
Exposure time: 9.3 s

Associated Targets(Human)

KAT2B Tchem Histone acetyltransferase KAT2B (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
KAT2B Tchem Histone acetyltransferase PCAF (884 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
KAT2B Tchem Histone acetyltransferase KAT2A/KAT2B (1 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
KAT2B Tchem Protein cereblon/Histone acetyltransferase KAT2B (3 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303368Certificate of AnalysisMar 19, 2024 Ab111758
ZJ24F0303369Certificate of AnalysisMar 19, 2024 Ab111758

Related Documents

 SDS

 Specification Sheet

FAQs and Articles

FAQ
Guidelines of Primary Antibodies for Western Blotting
Categories: Protocols
Tags:
Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls
Protocol of Western Blotting For High Molecular Weights
Categories: Protocols
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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

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46. Huq, M D Mostaqul MD, Gupta, Pawan P, Tsai, Nien-Pei NP and Wei, Li-Na LN..  (2006)  Modulation of testicular receptor 4 activity by mitogen-activated protein kinase-mediated phosphorylation..  Molecular & cellular proteomics : MCP,      [PMID:16887930]
47. Shin, Sook S and Janknecht, Ralf R..  (2007)  Concerted activation of the Mdm2 promoter by p72 RNA helicase and the coactivators p300 and P/CAF..  Journal of cellular biochemistry,    (1):   [PMID:17226766]
48. Wiper-Bergeron, Nadine N, Salem, Houssein Abdou HA, Tomlinson, Julianna J JJ, Wu, Dongmei D and Haché, Robert J G RJ..  (2007)  Glucocorticoid-stimulated preadipocyte differentiation is mediated through acetylation of C/EBPbeta by GCN5..  Proceedings of the National Academy of Sciences of the United States of America,    (20):   [PMID:17301242]
49. Gururaj, Anupama E AE, Peng, Shaohua S, Vadlamudi, Ratna K RK and Kumar, Rakesh R..  (2007)  Estrogen induces expression of BCAS3, a novel estrogen receptor-alpha coactivator, through proline-, glutamic acid-, and leucine-rich protein-1 (PELP1)..  Molecular endocrinology (Baltimore, Md.),      [PMID:17505058]
50. Zhu, Ping P and 8 more authors..  (2007)  A histone H2A deubiquitinase complex coordinating histone acetylation and H1 dissociation in transcriptional regulation..  Molecular cell,    (17):   [PMID:17707232]
51. Pickard, Adam A, Wong, Ping-Pui PP and McCance, Dennis J DJ..  (2010)  Acetylation of Rb by PCAF is required for nuclear localization and keratinocyte differentiation..  Journal of cell science,    (1):   [PMID:20940255]
52. Xia, Peng P and 10 more authors..  (2012)  EB1 acetylation by P300/CBP-associated factor (PCAF) ensures accurate kinetochore-microtubule interactions in mitosis..  Proceedings of the National Academy of Sciences of the United States of America,    (9):   [PMID:23001180]
53. Lin, Ruiting R and 7 more authors..  (2013)  Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth..  Molecular cell,    (22):   [PMID:23932781]
54. Gong, Fade F and 11 more authors..  (2015)  Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination..  Genes & development,    (15):   [PMID:25593309]
55. Chen, Sifan S and 6 more authors..  (2016)  SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing..  Nature communications,    (12):   [PMID:26867678]
56. Fournier, Marjorie M and 11 more authors..  (2016)  KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification..  Nature communications,    (31):   [PMID:27796307]
57. Rainger, Joe J and 15 more authors..  (2017)  A recurrent de novo mutation in ACTG1 causes isolated ocular coloboma..  Human mutation,      [PMID:28493397]
58. Ghosh, Tushar K TK and 7 more authors..  (2018)  Acetylation of TBX5 by KAT2B and KAT2A regulates heart and limb development..  Journal of molecular and cellular cardiology,      [PMID:29174768]

Solution Calculators

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