A synthetic peptide derived from human KAT9/Elp3 (AA 1-547)
Positive Control
WB: HeLa, K562, Hep G2, Jurkat, MCF7 and HEK293 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human KAT9/Elp3 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. May also have a methyltransferase activity.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant KAT9/Elp3 Antibody (Ab111794) - Western Blot All lanes: Recombinant KAT9/Elp3 Antibody (Ab111794) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 49, 61 kDa Exposure time: 14.6 s
1.Hawkes, Nicola A NA and 11 more authors.. (2002) Purification and characterization of the human elongator complex.. The Journal of biological chemistry, (25):[PMID:11714725]
2.Kim, Jae-Hyun JH, Lane, William S WS and Reinberg, Danny D.. (2002) Human Elongator facilitates RNA polymerase II transcription through chromatin.. Proceedings of the National Academy of Sciences of the United States of America, (5):[PMID:11818576]
3.Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455]
4.Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19):[PMID:16421571]
5.Close, Pierre P and 11 more authors.. (2006) Transcription impairment and cell migration defects in elongator-depleted cells: implication for familial dysautonomia.. Molecular cell, (19):[PMID:16713582]
6.Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31):[PMID:17974005]
7.Simpson, Claire L CL and 29 more authors.. (2009) Variants of the elongator protein 3 (ELP3) gene are associated with motor neuron degeneration.. Human molecular genetics, (1):[PMID:18996918]
8.Creppe, Catherine C and 16 more authors.. (2009) Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin.. Cell, (6):[PMID:19185337]
9.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
10.Ahmad, Yasmeen; Boisvert, Francois-Michel; Lundberg, Emma; Uhlen, Mathias and Lamond, Angus I.. (2012) Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization.. Molecular & cellular proteomics : MCP, [PMID:22002106]
11.Close, Pierre P and 11 more authors.. (2012) DERP6 (ELP5) and C3ORF75 (ELP6) regulate tumorigenicity and migration of melanoma cells as subunits of Elongator.. The Journal of biological chemistry, (21):[PMID:22854966]
12.Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
13.Dong, Chunming C and 9 more authors.. (2015) The Elp2 subunit is essential for elongator complex assembly and functional regulation.. Structure (London, England : 1993), (2):[PMID:25960406]
14.Dalwadi, Udit U and Yip, Calvin K CK.. (2018) Structural insights into the function of Elongator.. Cellular and molecular life sciences : CMLS, [PMID:29332244]
15.Bento-Abreu, Andre A and 22 more authors.. (2018) Elongator subunit 3 (ELP3) modifies ALS through tRNA modification.. Human molecular genetics, (1):[PMID:29415125]
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