Recombinant; Rabbit anti Human KIF5B Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112126-10μl | 10μl | In stock | $69.90 | |
| Ab112126-50μl | 50μl | In stock | $199.90 | |
| Ab112126-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab112126-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human KIF5B Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant KIF5B Antibody - Primary antibody, specific to KIF5B, Rabbit IgG |
|---|---|
| Synonyms | Conventional kinesin heavy chain | Ubiquitous kinesin heavy chain | UKHC | Conventional kinesin heavy chain | Kinesin 1 (110-120kD) | Kinesin family member 5B | Kinesin heavy chain | Kinesin heavy chain ubiquitous | Kinesin-1 heavy chain | KINH | KINH_HUM |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | KIF5B |
| Immunogen | A synthetic peptide derived from human KIF5B (AA 16-29) |
| Positive Control | WB: Mouse brain, HeLa, K562, MCF7, Jurkat, Hep G2, NIH/3T3 and C6 lysates. IHC: Human breast cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human KIF5B Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant KIF5B Antibody (Ab112126) - Western Blot
All lanes: Recombinant KIF5B Antibody (Ab112126) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 117 kDa
Exposure time: 25.7 s
Recombinant KIF5B Antibody (Ab112126) - IF/ICC
Immunocytochemistry analysis of KIF5B (green) in HeLa using Recombinant KIF5B Antibody (Ab112126), and cells were counterstained with DAPI (blue).
Recombinant KIF5B Antibody (Ab112126) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant KIF5B Antibody (Ab112126). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506085 | Certificate of Analysis | May 24, 2024 | Ab112126 |
| ZJ24F0506084 | Certificate of Analysis | May 24, 2024 | Ab112126 |
| 1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. James A D Good,Fang Wang,Oliver Rath,Hung Yi Kristal Kaan,Sandeep K Talapatra,Dawid Podgórski,Simon P MacKay,Frank Kozielski. (2013-02-12) Optimized S-trityl-L-cysteine-based inhibitors of kinesin spindle protein with potent in vivo antitumor activity in lung cancer xenograft models.. Journal of medicinal chemistry, 56 ((5)): (1878-1893). [PMID:23394180] |
| 4. Song M.. (2015) Progress in Discovery of KIF5B-RET Kinase Inhibitors for the Treatment of Non-Small-Cell Lung Cancer.. J Med Chem, 58 (9): (3672-3681). [PMID:25625428] |
| 5. Newton R, Bowler KA, Burns EM, Chapman PJ, Fairweather EE, Fritzl SJ, Goldberg KM, Hamilton NM, Holt SV, Hopkins GV, Jones SD, Jordan AM, Lyons AJ, Nikki March H, McDonald NQ, Maguire LA, Mould DP, Purkiss AG, Small HF, Stowell AI, Thomson GJ, Waddell ID, Waszkowycz B, Watson AJ, Ogilvie DJ.. (2016) The discovery of 2-substituted phenol quinazolines as potent RET kinase inhibitors with improved KDR selectivity.. Eur J Med Chem, 112 (20-32). [PMID:26874741] |
| 6. Jordan AM, Begum H, Fairweather E, Fritzl S, Goldberg K, Hopkins GV, Hamilton NM, Lyons AJ, March HN, Newton R, Small HF, Vishwanath S, Waddell ID, Waszkowycz B, Watson AJ, Ogilvie DJ.. (2016) Anilinoquinazoline inhibitors of the RET kinase domain-Elaboration of the 7-position.. Bioorg Med Chem Lett, 26 (11): (2724-2729). [PMID:27086121] |
| 7. Mathison CJN, Chianelli D, Rucker PV, Nelson J, Roland J, Huang Z, Yang Y, Jiang J, Xie YF, Epple R, Bursulaya B, Lee C, Gao MY, Shaffer J, Briones S, Sarkisova Y, Galkin A, Li L, Li N, Li C, Hua S, Kasibhatla S, Kinyamu-Akunda J, Kikkawa R, Molteni V, Tellew JE.. (2020) Efficacy and Tolerability of Pyrazolo[1,5-a]pyrimidine RET Kinase Inhibitors for the Treatment of Lung Adenocarcinoma.. ACS Med Chem Lett, 11 (4): (558-565). [PMID:32292564] |
| 8. Li X,Su J,Yang Y,Lian W,Deng Z,Yang Z,Chen G,Zhang B,Dong C,Liu X,Li L,Wang Z,Hu Z,Xu Q,Deng X. (2020) Discovery of 4-methyl-N-(4-((4-methylpiperazin- 1-yl)methyl)-3-(trifluoromethyl)phenyl)-3-((6-(pyridin-3-yl)-1H-pyrazolo[3,4-d]pyrimidin-4-yl)-oxy)benzamide as a potent inhibitor of RET and its gatekeeper mutant.. Eur J Med Chem, 207 (112755-112755). [PMID:32882611] |
| 9. Navone, F F and 6 more authors.. (1992) Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells.. The Journal of cell biology, [PMID:1607388] |
| 10. Kull, F J FJ, Sablin, E P EP, Lau, R R, Fletterick, R J RJ and Vale, R D RD.. (1996) Crystal structure of the kinesin motor domain reveals a structural similarity to myosin.. Nature, (11): [PMID:8606779] |
| 11. Sindelar, Charles V CV and 5 more authors.. (2002) Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy.. Nature structural biology, [PMID:12368902] |
| 12. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054] |
| 13. Su, Qingning Q, Cai, Qian Q, Gerwin, Claudia C, Smith, Carolyn L CL and Sheng, Zu-Hang ZH.. (2004) Syntabulin is a microtubule-associated protein implicated in syntaxin transport in neurons.. Nature cell biology, [PMID:15459722] |
| 14. Boucrot, Emmanuel E, Henry, Thomas T, Borg, Jean-Paul JP, Gorvel, Jean-Pierre JP and Méresse, Stéphane S.. (2005) The intracellular fate of Salmonella depends on the recruitment of kinesin.. Science (New York, N.Y.), (20): [PMID:15905402] |
| 15. Sindelar, Charles V and Downing, Kenneth H.. (2007) The beginning of kinesin's force-generating cycle visualized at 9-A resolution.. The Journal of cell biology, (7): [PMID:17470637] |
| 16. Splinter, Daniël D and 15 more authors.. (2010) Bicaudal D2, dynein, and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry.. PLoS biology, (6): [PMID:20386726] |
| 17. Gorbea, Carlos C and 6 more authors.. (2010) A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components.. The Journal of biological chemistry, (8): [PMID:20682791] |
| 18. Rosa-Ferreira, Cláudia C and Munro, Sean S.. (2011) Arl8 and SKIP act together to link lysosomes to kinesin-1.. Developmental cell, (13): [PMID:22172677] |
| 19. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 20. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 21. Seamster, Pamela E and 6 more authors.. (2012) Quantitative measurements and modeling of cargo-motor interactions during fast transport in the living axon.. Physical biology, [PMID:23011729] |
| 22. Tuli, Amit A and 9 more authors.. (2013) Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity.. Molecular biology of the cell, [PMID:24088571] |
| 23. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 24. Pekkurnaz, Gulcin G, Trinidad, Jonathan C JC, Wang, Xinnan X, Kong, Dong D and Schwarz, Thomas L TL.. (2014) Glucose regulates mitochondrial motility via Milton modification by O-GlcNAc transferase.. Cell, (3): [PMID:24995978] |
| 25. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
