Recombinant; Rabbit anti Human KIFC1 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112142-10μl | 10μl | In stock | $69.90 | |
| Ab112142-50μl | 50μl | In stock | $199.90 | |
| Ab112142-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab112142-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human KIFC1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant KIFC1 Antibody - Primary antibody, specific to KIFC1, Rabbit IgG |
|---|---|
| Synonyms | Kinesin-like protein 2 | Kinesin-related protein HSET | HSET KNSL2 | KIF C1 | KIFC 1 | KIFC1 | KIFC1_HUMAN | Kinesin family member C1 | Kinesin like 2 | Kinesin like protein 2 | Kinesin like protein KIFC1 | Kinesin related protein HSET | Kinesin-like prot |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | KIFC1 |
| Immunogen | A synthetic peptide derived from human KIFC1 (AA 17-30) |
| Positive Control | WB: K562, HeLa, MCF7 and Jurkat cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human KIFC1 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant KIFC1 Antibody (Ab112142) - Western Blot
All lanes: Recombinant KIFC1 Antibody (Ab112142) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 76 kDa
Exposure time: 13.9 s
Recombinant KIFC1 Antibody (Ab112142) - IF/ICC
Immunocytochemistry analysis of KIFC1 (green) in HeLa using Recombinant KIFC1 Antibody (Ab112142), and DAPI (blue).
Recombinant KIFC1 Antibody (Ab112142) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant KIFC1 Antibody (Ab112142). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506087 | Certificate of Analysis | May 24, 2024 | Ab112142 |
| ZJ24F0506086 | Certificate of Analysis | May 24, 2024 | Ab112142 |
| 1. Yang B, Lamb ML, Zhang T, Hennessy EJ, Grewal G, Sha L, Zambrowski M, Block MH, Dowling JE, Su N, Wu J, Deegan T, Mikule K, Wang W, Kaspera R, Chuaqui C, Chen H.. (2014) Discovery of potent KIFC1 inhibitors using a method of integrated high-throughput synthesis and screening.. J Med Chem, 57 (23): (9958-9970). [PMID:25458601] |
| 2. Dumas ME, Chen GY, Kendrick ND, Xu G, Larsen SD, Jana S, Waterson AG, Bauer JA, Hancock W, Sulikowski GA, Ohi R.. (2019) Dual inhibition of Kif15 by oxindole and quinazolinedione chemical probes.. Bioorg Med Chem Lett, 29 (2): (148-154). [PMID:30528696] |
| 3. Ando, A A and 9 more authors.. (1994) Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region.. Immunogenetics, [PMID:8276466] |
| 4. Janitz, K K and 6 more authors.. (1999) Genomic organization of the HSET locus and the possible association of HLA-linked genes with immotile cilia syndrome (ICS).. Immunogenetics, [PMID:10369922] |
| 5. Zhu, Changjun C and 7 more authors.. (2005) Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference.. Molecular biology of the cell, [PMID:15843429] |
| 6. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
