Recombinant KIFC1 Antibody - Primary antibody, specific to KIFC1, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab112142
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab112142-10μl
10μl
In stock
$69.90
Ab112142-50μl
50μl
In stock
$199.90
Ab112142-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab112142-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human KIFC1 Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant KIFC1 Antibody - Primary antibody, specific to KIFC1, Rabbit IgG
SynonymsKinesin-like protein 2 | Kinesin-related protein HSET | HSET KNSL2 | KIF C1 | KIFC 1 | KIFC1 | KIFC1_HUMAN | Kinesin family member C1 | Kinesin like 2 | Kinesin like protein 2 | Kinesin like protein KIFC1 | Kinesin related protein HSET | Kinesin-like prot
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityKIFC1
ImmunogenA synthetic peptide derived from human KIFC1 (AA 17-30)
Positive ControlWB: K562, HeLa, MCF7 and Jurkat cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human KIFC1 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant KIFC1 Antibody (Ab112142) - Western Blot
All lanes: Recombinant KIFC1 Antibody (Ab112142) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 76 kDa
Exposure time: 13.9 s

Recombinant KIFC1 Antibody (Ab112142) - IF/ICC
Immunocytochemistry analysis of KIFC1 (green) in HeLa using Recombinant KIFC1 Antibody (Ab112142), and DAPI (blue).

Recombinant KIFC1 Antibody (Ab112142) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant KIFC1 Antibody (Ab112142). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

KIFC1 Tchem Kinesin-like protein KIFC1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IF/ICC 1/50-1/200

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1/50-1/200

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506087Certificate of AnalysisMay 24, 2024 Ab112142
ZJ24F0506086Certificate of AnalysisMay 24, 2024 Ab112142

Related Documents

References

1. Yang B, Lamb ML, Zhang T, Hennessy EJ, Grewal G, Sha L, Zambrowski M, Block MH, Dowling JE, Su N, Wu J, Deegan T, Mikule K, Wang W, Kaspera R, Chuaqui C, Chen H..  (2014)  Discovery of potent KIFC1 inhibitors using a method of integrated high-throughput synthesis and screening..  J Med Chem,  57  (23): (9958-9970).  [PMID:25458601] [10.1021/jm501179r]
2. Dumas ME, Chen GY, Kendrick ND, Xu G, Larsen SD, Jana S, Waterson AG, Bauer JA, Hancock W, Sulikowski GA, Ohi R..  (2019)  Dual inhibition of Kif15 by oxindole and quinazolinedione chemical probes..  Bioorg Med Chem Lett,  29  (2): (148-154).  [PMID:30528696] [10.1016/j.bmcl.2018.12.008]
3. Ando, A A and 9 more authors..  (1994)  Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region..  Immunogenetics,      [PMID:8276466]
4. Janitz, K K and 6 more authors..  (1999)  Genomic organization of the HSET locus and the possible association of HLA-linked genes with immotile cilia syndrome (ICS)..  Immunogenetics,      [PMID:10369922]
5. Zhu, Changjun C and 7 more authors..  (2005)  Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference..  Molecular biology of the cell,      [PMID:15843429]
6. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
7. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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