| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112249-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab112249-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab112249-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab112249-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human KLF4 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant KLF4 Antibody |
|---|---|
| Synonyms | Endothelial Kruppel like zinc finger protein | EZF | GKLF | KLF | Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) | Kruppel like factor 4 (gut) | Krueppel-like factor 4 | KLF 4 | KLF-4 | Krueppel like factor |
| Specifications & Purity | Recombinant, ExactAb™, Validated, Lot by Lot |
| Biochemical and Physiological Mechanisms | Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryo |
| Host species | Rabbit |
| Specificity | KLF4 |
| Immunogen | A synthetic peptide derived from human KLF4 (AA 328-355) |
| Positive Control | WB: HeLa, Jurkat, MCF-7, A431, NIH/3T3 and C6 cell lysates. IHC: Mouse liver tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human KLF4 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant KLF4 Antibody (Ab112249) - Western Blot
All lanes: Recombinant KLF4 Antibody (Ab112249) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 50 kDa
Exposure time: 100.0 s
Recombinant KLF4 Antibody (Ab112249) - Western Blot
All lanes: Recombinant KLF4 Antibody (Ab112249) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 50 kDa
Exposure time: 17.4 s
Recombinant KLF4 Antibody (Ab112249) - IHC
Immunohistochemical analysis of paraffin-embedded mouse liver, using Recombinant KLF4 Antibody (Ab112249).
Recombinant KLF4 Antibody (Ab112249) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant KLF4 Antibody (Ab112249).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 "> 1/100 - 1/200 |
| IF/ICC | 1/50 - 1/200 "> 1/50 - 1/200 |
| WB |
1/1000-1/2000
">
1/1000-1/2000
|
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