Recombinant; Rabbit anti Human Lamin B2 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112672-10μl | 10μl | In stock | $66.90 | |
| Ab112672-50μl | 50μl | In stock | $199.90 | |
| Ab112672-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab112672-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human Lamin B2 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant Lamin B2 Antibody - Primary antibody, specific to LMNB2, Rabbit IgG |
|---|---|
| Synonyms | LAMB 2; LAMB2; Lamin-B2; LMN 2; LMN B2; LMN2; LMNB 2; LMNB2; LMNB2_HUMAN; MGC2721; LAMB 2 antibody; LAMB2 antibody; Lamin-B2 antibody; LMN 2 antibody; LMN B2 antibody; LMN2 antibody; LMNB 2 antibody; LMNB2 antibody; LMNB2_HUMAN antibody; MGC2721 antibody; |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | LMNB2 |
| Immunogen | A synthetic peptide derived from human Lamin B2 (AA 582-597) |
| Positive Control | WB: Raji and HeLa cell lysates. ICC/IF: HeLa cells. IHC: Human lung cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Lamin B2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Lamin B2 Antibody (Ab112672) - Western Blot
All lanes: Recombinant Lamin B2 Antibody (Ab112672) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa
Exposure time: 120.0 s
Recombinant Lamin B2 Antibody (Ab112672) - Western Blot
All lanes: Recombinant Lamin B2 Antibody (Ab112672) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa
Exposure time: 30.0 s
Recombinant Lamin B2 Antibody (Ab112672) - IF/ICC
Immunocytochemistry analysis of Lamin B2 (green) in HeLa using Recombinant Lamin B2 Antibody (Ab112672), and cells were counterstained with DAPI (blue).
Recombinant Lamin B2 Antibody (Ab112672) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Lamin B2 Antibody (Ab112672). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303893 | Certificate of Analysis | Apr 02, 2024 | Ab112672 |
| ZJ24F0303892 | Certificate of Analysis | Apr 02, 2024 | Ab112672 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Biamonti, G G and 9 more authors.. (1992) The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.. Molecular and cellular biology, [PMID:1630457] |
| 4. Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1): [PMID:15057824] |
| 5. Hegele, Robert A RA and 6 more authors.. (2006) Sequencing of the reannotated LMNB2 gene reveals novel mutations in patients with acquired partial lipodystrophy.. American journal of human genetics, [PMID:16826530] |
| 6. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 7. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 8. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 9. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 10. Gao, Jinzhi J, Li, Yuanyuan Y, Fu, Xi X and Luo, Xiaoping X.. (2012) A Chinese patient with acquired partial lipodystrophy caused by a novel mutation with LMNB2 gene.. Journal of pediatric endocrinology & metabolism : JPEM, [PMID:22768673] |
| 11. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 12. Damiano, John A JA and 19 more authors.. (2015) Mutation of the nuclear lamin gene LMNB2 in progressive myoclonus epilepsy with early ataxia.. Human molecular genetics, (15): [PMID:25954030] |
| 13. Lek, Monkol and 79 more authors.. (2016) Analysis of protein-coding genetic variation in 60,706 humans.. Nature, (18): [PMID:27535533] |
| 14. Parry, David A and 17 more authors.. (2021) Heterozygous lamin B1 and lamin B2 variants cause primary microcephaly and define a novel laminopathy.. Genetics in medicine : official journal of the American College of Medical Genetics, [PMID:33033404] |
| 15. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
