| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112746-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab112746-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $169.90 | |
| Ab112746-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $249.90 | |
| Ab112746-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
Recombinant; Rabbit anti Human LAMP2 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant LAMP2 Antibody - Primary antibody, specific to LAMP2, Rabbit IgG |
|---|---|
| Synonyms | Anti-LAMP2 antibody | CD107 antigen like family member B antibody | CD107 antigen-like family member B antibody | CD107b antibody | LAMP 2 antibody | LAMP 2C antibody | LAMP-2 antibody | LAMP2 antibody | LAMP2_HUMAN antibody | LAMPB antibody | LGP 96 anti |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | LAMP2 |
| Immunogen | Recombinant protein of human LAMP2 (AA 29-192). |
| Positive Control | WB: HeLa, A549, Jurkat and THP-1 cell lysates. IHC: Human lung cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human LAMP2 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant LAMP2 Antibody (Ab112746) - Western Blot
All lanes: Recombinant LAMP2 Antibody (Ab112746) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 110-130 kDa
Observed band size: 106 kDa
Exposure time: 3.2 s
Recombinant LAMP2 Antibody (Ab112746) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant LAMP2 Antibody (Ab112746). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001479 | Certificate of Analysis | Nov 02, 2023 | Ab112746 |
| ZJ23F1001480 | Certificate of Analysis | Nov 02, 2023 | Ab112746 |
| 1. Demirel Ö, Jan I, Wolters D, Blanz J, Saftig P, Tampé R, Abele R. (2012) The lysosomal polypeptide transporter TAPL is stabilized by interaction with LAMP-1 and LAMP-2.. J Cell Sci, 125 (Pt 18): (4230-40). [PMID:22641697] |
| 2. Peng Li,Min Ji,Fei Lu,Jingru Zhang,Huanjie Li,Taixing Cui,Xing Li Wang,Dongqi Tang,Chunyan Ji. (2014-06-01) Degradation of AF1Q by chaperone-mediated autophagy.. Experimental cell research, 327 ((1)): (48-56). [PMID:24880125] |
| 3. Carlsson, S R SR and Fukuda, M M.. (1990) The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones.. The Journal of biological chemistry, (25): [PMID:2243102] |
| 4. Mane, S M SM and 6 more authors.. (1989) Purification and characterization of human lysosomal membrane glycoproteins.. Archives of biochemistry and biophysics, [PMID:2912382] |
| 5. Fukuda, M M, Viitala, J J, Matteson, J J and Carlsson, S R SR.. (1988) Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences.. The Journal of biological chemistry, (15): [PMID:3198605] |
| 6. Konecki, D S DS, Foetisch, K K, Zimmer, K P KP, Schlotter, M M and Lichter-Konecki, U U.. (1995) An alternatively spliced form of the human lysosome-associated membrane protein-2 gene is expressed in a tissue-specific manner.. Biochemical and biophysical research communications, (13): [PMID:7488019] |
| 7. Konecki, D S DS, Foetisch, K K, Schlotter, M M and Lichter-Konecki, U U.. (1994) Complete cDNA sequence of human lysosome-associated membrane protein-2.. Biochemical and biophysical research communications, (30): [PMID:7999007] |
| 8. Carlsson, S R SR, Lycksell, P O PO and Fukuda, M M.. (1993) Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2.. Archives of biochemistry and biophysics, [PMID:8323299] |
| 9. Sawada, R R, Jardine, K A KA and Fukuda, M M.. (1993) The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes.. The Journal of biological chemistry, (25): [PMID:8517882] |
| 10. Cuervo, A M AM and Dice, J F JF.. (1996) A receptor for the selective uptake and degradation of proteins by lysosomes.. Science (New York, N.Y.), (26): [PMID:8662539] |
| 11. Zhang, Hui H, Li, Xiao-Jun XJ, Martin, Daniel B DB and Aebersold, Ruedi R.. (2003) Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.. Nature biotechnology, [PMID:12754519] |
| 12. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 13. Arad, Michael M and 10 more authors.. (2005) Glycogen storage diseases presenting as hypertrophic cardiomyopathy.. The New England journal of medicine, (27): [PMID:15673802] |
| 14. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651] |
| 15. Musumeci, Olimpia O and 9 more authors.. (2005) Asymptomatic hyperCKemia in a case of Danon disease due to a missense mutation in Lamp-2 gene.. Neuromuscular disorders : NMD, [PMID:15907287] |
| 16. Eskelinen, Eeva-Liisa EL and 9 more authors.. (2005) Unifying nomenclature for the isoforms of the lysosomal membrane protein LAMP-2.. Traffic (Copenhagen, Denmark), [PMID:16190986] |
| 17. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 18. Schröder, Bernd B and 8 more authors.. (2007) Integral and associated lysosomal membrane proteins.. Traffic (Copenhagen, Denmark), [PMID:17897319] |
| 19. Crotzer, Victoria L VL, Glosson, Nicole N, Zhou, Delu D, Nishino, Ichizo I and Blum, Janice S JS.. (2010) LAMP-2-deficient human B cells exhibit altered MHC class II presentation of exogenous antigens.. Immunology, [PMID:20518820] |
| 20. Rout, Ashok K AK, Strub, Marie-Paule MP, Piszczek, Grzegorz G and Tjandra, Nico N.. (2014) Structure of transmembrane domain of lysosome-associated membrane protein type 2a (LAMP-2A) reveals key features for substrate specificity in chaperone-mediated autophagy.. The Journal of biological chemistry, (19): [PMID:25342746] |
| 21. Pérez, Liliana L and 5 more authors.. (2016) LAMP-2C Inhibits MHC Class II Presentation of Cytoplasmic Antigens by Disrupting Chaperone-Mediated Autophagy.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:26856698] |
| 22. Hubert, Virginie V and 5 more authors.. (2016) LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes.. Biology open, (15): [PMID:27628032] |
| 23. Ueo, Ayako and 5 more authors.. (2020) Lysosome-Associated Membrane Proteins Support the Furin-Mediated Processing of the Mumps Virus Fusion Protein.. Journal of virology, (1): [PMID:32295904] |
| 24. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
