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Recombinant LAMP2 Antibody - Primary antibody, specific to LAMP2, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.8 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human LAMP2 Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
    Application:
  • IHC
  • WB
Item Number
Ab112746
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab112746-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$56.90
Ab112746-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$169.90
Ab112746-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$249.90
Ab112746-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,199.90

Recombinant; Rabbit anti Human LAMP2 Antibody; WB, IHC; Unconjugated

View related series
Accession#:P13473 Gene ID:3920 LAMP2

Basic Description

Product NameRecombinant LAMP2 Antibody - Primary antibody, specific to LAMP2, Rabbit IgG
SynonymsAnti-LAMP2 antibody; CD107 antigen like family member B antibody; CD107 antigen-like family member B antibody; CD107b antibody; LAMP 2 antibody; LAMP 2C antibody; LAMP-2 antibody; LAMP2 antibody; LAMP2_HUMAN antibody; LAMPB antibody; LGP 96 antibody; LGP1
Specifications & PurityExactAb™, Validated, Recombinant, 0.8 mg/mL
Host speciesRabbit
SpecificityLAMP2
ImmunogenRecombinant protein of human LAMP2 (AA 29-192).
Positive ControlWB: HeLa, A549, Jurkat and THP-1 cell lysates. IHC: Human lung cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human LAMP2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500 - 1/1000
IHC : 1/50 - 1/100
Protein Function
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Post-translational
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.8 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant LAMP2 Antibody (Ab112746) - Western Blot
All lanes: Recombinant LAMP2 Antibody (Ab112746) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 110-130 kDa
Observed band size: 106 kDa
Exposure time: 3.2 s

Recombinant LAMP2 Antibody (Ab112746) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant LAMP2 Antibody (Ab112746). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

LAMP2 Tbio Lysosome-associated membrane glycoprotein 2 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001479Certificate of AnalysisNov 02, 2023 Ab112746
ZJ23F1001480Certificate of AnalysisNov 02, 2023 Ab112746

Related Documents

References

1. Demirel Ö, Jan I, Wolters D, Blanz J, Saftig P, Tampé R, Abele R.  (2012)  The lysosomal polypeptide transporter TAPL is stabilized by interaction with LAMP-1 and LAMP-2..  J Cell Sci,  125  (Pt 18): (4230-40).  [PMID:22641697]
2. Peng Li,Min Ji,Fei Lu,Jingru Zhang,Huanjie Li,Taixing Cui,Xing Li Wang,Dongqi Tang,Chunyan Ji.  (2014-06-01)  Degradation of AF1Q by chaperone-mediated autophagy..  Experimental cell research,  327  ((1)): (48-56).  [PMID:24880125]
3. Carlsson, S R SR and Fukuda, M M..  (1990)  The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones..  The Journal of biological chemistry,    (25):   [PMID:2243102]
4. Mane, S M SM and 6 more authors..  (1989)  Purification and characterization of human lysosomal membrane glycoproteins..  Archives of biochemistry and biophysics,      [PMID:2912382]
5. Fukuda, M M, Viitala, J J, Matteson, J J and Carlsson, S R SR..  (1988)  Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences..  The Journal of biological chemistry,    (15):   [PMID:3198605]
6. Konecki, D S DS, Foetisch, K K, Zimmer, K P KP, Schlotter, M M and Lichter-Konecki, U U..  (1995)  An alternatively spliced form of the human lysosome-associated membrane protein-2 gene is expressed in a tissue-specific manner..  Biochemical and biophysical research communications,    (13):   [PMID:7488019]
7. Konecki, D S DS, Foetisch, K K, Schlotter, M M and Lichter-Konecki, U U..  (1994)  Complete cDNA sequence of human lysosome-associated membrane protein-2..  Biochemical and biophysical research communications,    (30):   [PMID:7999007]
8. Carlsson, S R SR, Lycksell, P O PO and Fukuda, M M..  (1993)  Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2..  Archives of biochemistry and biophysics,      [PMID:8323299]
9. Sawada, R R, Jardine, K A KA and Fukuda, M M..  (1993)  The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes..  The Journal of biological chemistry,    (25):   [PMID:8517882]
10. Cuervo, A M AM and Dice, J F JF..  (1996)  A receptor for the selective uptake and degradation of proteins by lysosomes..  Science (New York, N.Y.),    (26):   [PMID:8662539]
11. Zhang, Hui H, Li, Xiao-Jun XJ, Martin, Daniel B DB and Aebersold, Ruedi R..  (2003)  Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry..  Nature biotechnology,      [PMID:12754519]
12. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,      [PMID:15340161]
13. Arad, Michael M and 10 more authors..  (2005)  Glycogen storage diseases presenting as hypertrophic cardiomyopathy..  The New England journal of medicine,    (27):   [PMID:15673802]
14. Ross, Mark T MT and 281 more authors..  (2005)  The DNA sequence of the human X chromosome..  Nature,    (17):   [PMID:15772651]
15. Musumeci, Olimpia O and 9 more authors..  (2005)  Asymptomatic hyperCKemia in a case of Danon disease due to a missense mutation in Lamp-2 gene..  Neuromuscular disorders : NMD,      [PMID:15907287]
16. Eskelinen, Eeva-Liisa EL and 9 more authors..  (2005)  Unifying nomenclature for the isoforms of the lysosomal membrane protein LAMP-2..  Traffic (Copenhagen, Denmark),      [PMID:16190986]
17. Liu, Tao T and 6 more authors..  ()  Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry..  Journal of proteome research,      [PMID:16335952]
18. Schröder, Bernd B and 8 more authors..  (2007)  Integral and associated lysosomal membrane proteins..  Traffic (Copenhagen, Denmark),      [PMID:17897319]
19. Crotzer, Victoria L VL, Glosson, Nicole N, Zhou, Delu D, Nishino, Ichizo I and Blum, Janice S JS..  (2010)  LAMP-2-deficient human B cells exhibit altered MHC class II presentation of exogenous antigens..  Immunology,      [PMID:20518820]
20. Rout, Ashok K AK, Strub, Marie-Paule MP, Piszczek, Grzegorz G and Tjandra, Nico N..  (2014)  Structure of transmembrane domain of lysosome-associated membrane protein type 2a (LAMP-2A) reveals key features for substrate specificity in chaperone-mediated autophagy..  The Journal of biological chemistry,    (19):   [PMID:25342746]
21. Pérez, Liliana L and 5 more authors..  (2016)  LAMP-2C Inhibits MHC Class II Presentation of Cytoplasmic Antigens by Disrupting Chaperone-Mediated Autophagy..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:26856698]
22. Hubert, Virginie V and 5 more authors..  (2016)  LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes..  Biology open,    (15):   [PMID:27628032]
23. Ueo, Ayako and 5 more authors..  (2020)  Lysosome-Associated Membrane Proteins Support the Furin-Mediated Processing of the Mumps Virus Fusion Protein..  Journal of virology,    (1):   [PMID:32295904]

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