Recombinant; Rabbit anti Human LAT Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112829-10μl | 10μl | In stock | $66.90 | |
| Ab112829-50μl | 50μl | In stock | $199.90 | |
| Ab112829-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab112829-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human LAT Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant LAT Antibody - Primary antibody, specific to LAT, Rabbit IgG |
|---|---|
| Synonyms | 36 kDa phospho tyrosine adapter protein antibody; 36 kDa phospho-tyrosine adapter protein antibody; 36 kDa phospho-tyrosine adaptor protein antibody; LAT 1 antibody; lat antibody; LAT_HUMAN antibody; LAT1 antibody; Linker for activation of T cellsantibody |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | LAT |
| Immunogen | A synthetic peptide derived from human LAT (AA 217-234). |
| Positive Control | WB: Jurkat and MOLT-4 cell lysates. IHC: Human tonsil tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human LAT Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant LAT Antibody (Ab112829) - Western Blot
All lanes: Recombinant LAT Antibody (Ab112829) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 24 kDa
Exposure time: 35.0 s
Recombinant LAT Antibody (Ab112829) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant LAT Antibody (Ab112829). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102612 | Certificate of Analysis | Jan 30, 2024 | Ab112829 |
| ZJ24F0102611 | Certificate of Analysis | Jan 30, 2024 | Ab112829 |
| 1. Chen IJ, Chen HL, Demetriou M.. (2007) Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling.. J Biol Chem, 282 (48): (35361-35372). [PMID:17897956] |
| 2. Zhang, W W, Sloan-Lancaster, J J, Kitchen, J J, Trible, R P RP and Samelson, L E LE.. (1998) LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.. Cell, (9): [PMID:9489702] |
| 3. Weber, J R JR and 8 more authors.. (1998) Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells.. The Journal of experimental medicine, (6): [PMID:9529333] |
| 4. Zhang, W W, Trible, R P RP and Samelson, L E LE.. (1998) LAT palmitoylation: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation.. Immunity, [PMID:9729044] |
| 5. Jevremovic, D D and 8 more authors.. (1999) Cutting edge: a role for the adaptor protein LAT in human NK cell-mediated cytotoxicity.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:10072481] |
| 6. Lindholm, C K CK, Gylfe, E E, Zhang, W W, Samelson, L E LE and Welsh, M M.. (1999) Requirement of the Src homology 2 domain protein Shb for T cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells.. The Journal of biological chemistry, (24): [PMID:10488157] |
| 7. Tridandapani, S S and 5 more authors.. (2000) The adapter protein LAT enhances fcgamma receptor-mediated signal transduction in myeloid cells.. The Journal of biological chemistry, (7): [PMID:10781611] |
| 8. Zhang, W W and 5 more authors.. (2000) Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling.. The Journal of biological chemistry, (28): [PMID:10811803] |
| 9. Asazuma, N N and 11 more authors.. (2000) Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxin.. The Journal of biological chemistry, (27): [PMID:10942756] |
| 10. Baker, J E JE, Majeti, R R, Tangye, S G SG and Weiss, A A.. (2001) Protein tyrosine phosphatase CD148-mediated inhibition of T-cell receptor signal transduction is associated with reduced LAT and phospholipase Cgamma1 phosphorylation.. Molecular and cellular biology, [PMID:11259588] |
| 11. Paz, P E PE and 5 more authors.. (2001) Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells.. The Biochemical journal, (1): [PMID:11368773] |
| 12. Perez-Villar, Juan J JJ and 8 more authors.. (2002) Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav.. Biochemistry, (27): [PMID:12186560] |
| 13. Lin, Joseph J and Weiss, Arthur A.. (2003) The tyrosine phosphatase CD148 is excluded from the immunologic synapse and down-regulates prolonged T cell signaling.. The Journal of cell biology, (18): [PMID:12913111] |
| 14. Sommers, Connie L CL, Samelson, Lawrence E LE and Love, Paul E PE.. (2004) LAT: a T lymphocyte adapter protein that couples the antigen receptor to downstream signaling pathways.. BioEssays : news and reviews in molecular, cellular and developmental biology, [PMID:14696041] |
| 15. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186] |
| 16. Martin, Joel J and 121 more authors.. (2004) The sequence and analysis of duplication-rich human chromosome 16.. Nature, (23): [PMID:15616553] |
| 17. Tedoldi, Sara S and 16 more authors.. (2006) Transmembrane adaptor molecules: a new category of lymphoid-cell markers.. Blood, (1): [PMID:16160011] |
| 18. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087] |
| 19. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 20. Xie, Ji-Ji JJ, Liang, Jia-Qi JQ, Diao, Liang-Hui LH, Altman, Amnon A and Li, Yingqiu Y.. (2013) TNFR-associated factor 6 regulates TCR signaling via interaction with and modification of LAT adapter.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:23514740] |
| 21. Yang, Yin Y and 11 more authors.. (2015) The Us3 Protein of Herpes Simplex Virus 1 Inhibits T Cell Signaling by Confining Linker for Activation of T Cells (LAT) Activation via TRAF6 Protein.. The Journal of biological chemistry, (19): [PMID:25907557] |
| 22. Keller, Baerbel B and 13 more authors.. (2016) Early onset combined immunodeficiency and autoimmunity in patients with loss-of-function mutation in LAT.. The Journal of experimental medicine, (27): [PMID:27242165] |
| 23. Bacchelli, Chiara C and 13 more authors.. (2017) Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency.. The Journal of allergy and clinical immunology, [PMID:27522155] |
