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Recombinant LEF1 Antibody - Primary antibody, specific to LEF1, Rabbit IgG

ExactAb™
Recombinant
Validated
0.24 mg/mL

Features and benefits

Short Overview:
Recombinant (MyR1-LEF1); Rabbit anti Human LEF1 Antibody; IHC; Unconjugated
Species reactivity(Reacts with): Human
Isotype: Rabbit IgG

Application:

Item Number

Ab112964

Grouped product items
SKU	Size	Availability	Price
Ab112964-10μl	10μl	In stock	$49.90
Ab112964-50μl	50μl	In stock	$159.90
Ab112964-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$229.90
Ab112964-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,199.90

Recombinant (MyR1-LEF1); Rabbit anti Human LEF1 Antibody; IHC; Unconjugated

View related series

Accession#:Q9UJU2 Gene ID:51176 LEF1

Basic Description

Product Name	Recombinant LEF1 Antibody - Primary antibody, specific to LEF1, Rabbit IgG
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.24 mg/mL
Host species	Rabbit
Specificity	LEF1
Positive Control	IHC: Human tonsil tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human LEF1 Antibody, Recombinant (MyR1-LEF1), could be used for IHC and so on. Application IHC: 1/100-1/200 Protein Function Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.24 mg/mL
Storage Temp	Store at 2-8°C,Freeze prohibited
Shipped In	Wet ice
Stability And Storage	Store at 2-8°C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant LEF1 Antibody (Ab112964) - IHC
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue labelling LEF1 with Recombinant LEF1 Antibody (Ab112964) at 1/200 dilution. The section was incubated with Ab112964 for 30 mins at room temperature. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval.

Application

Dilution info

IHC

1/100 - 1/200

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505264	Certificate of Analysis	May 09, 2024	Ab112964
ZJ24F0505263	Certificate of Analysis	May 09, 2024	Ab112964

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