Recombinant LIN28B Antibody - Primary antibody, specific to LIN28B, Rabbit IgG

    Application:
  • IF/ICC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab113176
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab113176-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab113176-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
Ab113176-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab113176-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,799.90

Recombinant; Rabbit anti Human LIN28B Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant LIN28B Antibody - Primary antibody, specific to LIN28B, Rabbit IgG
SynonymsCSDD 2 antibody | CSDD2antibody | FLJ16517 antibody | Lin 28 homolog B (C. elegans) antibody | Lin 28.2 antibody | Lin-28B antibody | LIN28B antibody | LN28B_HUMAN antibody | Protein lin-28 homolog B antibody | CSDD2 | CSDD2protein lin-28 homolog B | FLJ1
Specifications & PurityExactAb™, Validated, Recombinant, 0.6 mg/mL
Host speciesRabbit
SpecificityLIN28B
ImmunogenA synthetic peptide derived from human Lin28B (AA 1-250)
Positive ControlWB: HeLa, Hep G2 and HEK293 cell lysates. ICC/IF: MCF7 cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human LIN28B Antibody, Recombinant, could be used for WB, ICC/IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Function
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.6 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant LIN28B Antibody (Ab113176) - Western Blot
All lanes: Recombinant LIN28B Antibody (Ab113176) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 33 kDa
Exposure time: 23.1 s

Recombinant LIN28B Antibody (Ab113176) - IF/ICC
Immunofluorescence analysis of LIN28B in MCF7 using Recombinant LIN28B Antibody (Ab113176).

Associated Targets(Human)

LIN28B Tbio Protein lin-28 homolog B (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
LIN28B Tbio Protein lin-28 homolog B (3 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IF/ICC 1/50-1/200

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1/50-1/200

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607527Certificate of AnalysisJun 27, 2024 Ab113176
ZJ24F0607526Certificate of AnalysisJun 27, 2024 Ab113176

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