Recombinant LIN28B Antibody - Primary antibody, specific to LIN28B, Rabbit IgG
Synonyms
CSDD 2 antibody | CSDD2antibody | FLJ16517 antibody | Lin 28 homolog B (C. elegans) antibody | Lin 28.2 antibody | Lin-28B antibody | LIN28B antibody | LN28B_HUMAN antibody | Protein lin-28 homolog B antibody | CSDD2 | CSDD2protein lin-28 homolog B | FLJ1
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species
Rabbit
Specificity
LIN28B
Immunogen
A synthetic peptide derived from human Lin28B (AA 1-250)
Positive Control
WB: HeLa, Hep G2 and HEK293 cell lysates. ICC/IF: MCF7 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human LIN28B Antibody, Recombinant, could be used for WB, ICC/IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Function Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.6 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant LIN28B Antibody (Ab113176) - Western Blot All lanes: Recombinant LIN28B Antibody (Ab113176) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 33 kDa Exposure time: 23.1 s
Recombinant LIN28B Antibody (Ab113176) - IF/ICC Immunofluorescence analysis of LIN28B in MCF7 using Recombinant LIN28B Antibody (Ab113176).