Recombinant, ExactAb™, KD Validation, Validated, See COA
Biochemical and Physiological Mechanisms
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts
Host species
Rabbit
Specificity
MBD1
Immunogen
A synthetic peptide derived from human MBD1 (AA 550-605).
Positive Control
WB: HeLa, MCF7, Hep G2, HEK293 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, KD Validation, Recombinant, Validated
Product Description
Rabbit anti Human MBD1 Antibody, Recombinant, could be used for WB and so on.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
See COA
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MBD1 Antibody (Ab114408) - Western Blot All lanes: Recombinant MBD1 Antibody (Ab114408) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (AP132P) at 1/20000 dilution
Predicted band size: 67 kDa Observed band size: 74, 81 kDa. Recombinant MBD1 Antibody (Ab114408) recognizes two isoforms of MBD1. Exposure time: 36.1 s
Recombinant MBD1 Antibody (Ab114408) - Western Blot All lanes use Recombinant MBD1 Antibody (Ab114408) at 1/1000 dilution for 1 hour at room temperature.
Recombinant MBD1 Antibody (Ab114408) - Western Blot All lanes: Recombinant MBD1 Antibody (Ab114408) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (AP132P) at 1/20000 dilution
Predicted band size: 67 kDa Observed band size: 74, 81 kDa. Recombinant MBD1 Antibody (Ab114408) recognizes two isoforms of MBD1. Exposure time: 36.1 s
Associated Targets(Human)
MBD1TbioMethyl-CpG-binding domain protein 1 (0 Activities)