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Recombinant MBD2 Antibody - Primary antibody, specific to MBD2, Rabbit IgG

Recombinant

Validated

ExactAb™

0.7 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human MBD2 Antibody; WB, IHC, IF, ICC; Unconjugated
Species reactivity(Reacts with): Human,Mouse,Rat
Isotype: Rabbit IgG

Application:

IF/ICC
IHC
WB

Item Number

Ab114412

Grouped product items
SKU	Size	Availability	Price
Ab114412-10μl	10μl	In stock	$89.90
Ab114412-50μl	50μl	In stock	$269.90
Ab114412-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$379.90
Ab114412-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90

Recombinant; Rabbit anti Human MBD2 Antibody; WB, IHC, IF, ICC; Unconjugated

View related series

Click to view target information: MBD2

Accession#: Q9UBB5 Gene ID: 8932 MBD2

Basic Description

Product Name	Recombinant MBD2 Antibody - Primary antibody, specific to MBD2, Rabbit IgG
Synonyms	Demethylase \| DMTase \| Methyl-CpG-binding protein MBD2 \| Demethylase \| DMTase \| Mbd2 \| MBD2a \| Demethylase antibody \| DMTase antibody \| MBD 2 antibody \| Mbd2 antibody \| MBD2_HUMAN antibody \| MBD2a antibody \| Methyl CpG binding protein MBD2 antibody \| Meth
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.7 mg/mL
Host species	Rabbit
Specificity	MBD2
Immunogen	A synthetic peptide derived from human MBD2 (AA 216-234)
Positive Control	WB: Rat brain, C6, 3T3, HeLa lysates. IHC: Human tonsil tissue. IF/ICC: HL-60 cells
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human MBD2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.7 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MBD2 Tchem Methyl-CpG-binding domain protein 2 (0 Activities)

Discover Target: MBD2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MBD2 Tchem Methyl-CpG-binding domain protein 2 (8 Activities)

Discover Target: MBD2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Application

Dilution info

1/500-1/1000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0506101	Certificate of Analysis	May 28, 2024	Ab114412
ZJ24F0506100	Certificate of Analysis	May 28, 2024	Ab114412

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
2. Hendrich, B B and Bird, A A.. (1998) Identification and characterization of a family of mammalian methyl-CpG binding proteins.. Molecular and cellular biology, [PMID:9774669]
3. Bhattacharya, S K SK, Ramchandani, S S, Cervoni, N N and Szyf, M M.. (1999) A mammalian protein with specific demethylase activity for mCpG DNA.. Nature, (18): [PMID:10050851]
4. Hendrich, B B and 5 more authors.. (1999) Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:10441743]
5. Ng, H H HH and 8 more authors.. (1999) MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex.. Nature genetics, [PMID:10471499]
6. Tatematsu, K I KI, Yamazaki, T T and Ishikawa, F F.. (2000) MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:10947852]
7. Boeke, J J, Ammerpohl, O O, Kegel, S S, Moehren, U U and Renkawitz, R R.. (2000) The minimal repression domain of MBD2b overlaps with the methyl-CpG-binding domain and binds directly to Sin3A.. The Journal of biological chemistry, (10): [PMID:10950960]
8. Humphrey, G W GW and 6 more authors.. (2001) Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.. The Journal of biological chemistry, (2): [PMID:11102443]
9. Sekimata, M M, Takahashi, A A, Murakami-Sekimata, A A and Homma, Y Y.. (2001) Involvement of a novel zinc finger protein, MIZF, in transcriptional repression by interacting with a methyl-CpG-binding protein, MBD2.. The Journal of biological chemistry, (16): [PMID:11553631]
10. Brackertz, Marc M, Boeke, Joern J, Zhang, Ru R and Renkawitz, Rainer R.. (2002) Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3.. The Journal of biological chemistry, (25): [PMID:12183469]
11. Lembo, Francesca F and 6 more authors.. (2003) MBDin, a novel MBD2-interacting protein, relieves MBD2 repression potential and reactivates transcription from methylated promoters.. Molecular and cellular biology, [PMID:12588985]
12. Fujita, Hidetoshi H and 5 more authors.. (2003) Antithetic effects of MBD2a on gene regulation.. Molecular and cellular biology, [PMID:12665568]
13. Jin, Seung-Gi SG, Jiang, Chun-Ling CL, Rauch, Tibor T, Li, Hongwei H and Pfeifer, Gerd P GP.. (2005) MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing.. The Journal of biological chemistry, (1): [PMID:15701600]
14. Brackertz, Marc M, Gong, Zihua Z, Leers, Jörg J and Renkawitz, Rainer R.. (2006) p66alpha and p66beta of the Mi-2/NuRD complex mediate MBD2 and histone interaction.. Nucleic acids research, [PMID:16415179]
15. Le Guezennec, Xavier X and 6 more authors.. (2006) MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties.. Molecular and cellular biology, [PMID:16428440]
16. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
17. Spruijt, Cornelia G CG and 6 more authors.. (2010) CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.. Molecular bioSystems, [PMID:20523938]
18. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
19. Cramer, Jason M JM and 5 more authors.. (2014) Probing the dynamic distribution of bound states for methylcytosine-binding domains on DNA.. The Journal of biological chemistry, (17): [PMID:24307175]
20. Spruijt, Cornelia G CG and 15 more authors.. (2016) ZMYND8 Co-localizes with NuRD on Target Genes and Regulates Poly(ADP-Ribose)-Dependent Recruitment of GATAD2A/NuRD to Sites of DNA Damage.. Cell reports, (11): [PMID:27732854]
21. Hoffmeister, Helen H and 12 more authors.. (2017) CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality.. Nucleic acids research, (13): [PMID:28977666]

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