Basic Description

Product Name	Recombinant MBD2 Antibody - Primary antibody, specific to MBD2, Rabbit IgG
Synonyms	Demethylase \| DMTase \| Methyl-CpG-binding protein MBD2 \| Demethylase \| DMTase \| Mbd2 \| MBD2a \| Demethylase antibody \| DMTase antibody \| MBD 2 antibody \| Mbd2 antibody \| MBD2_HUMAN antibody \| MBD2a antibody \| Methyl CpG binding protein MBD2 antibody \| Meth
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.7 mg/mL
Host species	Rabbit
Specificity	MBD2
Immunogen	A synthetic peptide derived from human MBD2 (AA 216-234)
Positive Control	WB: A375, MCF7, HeLa, Raji, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HL-60 cells
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human MBD2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.7 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MBD2 Tchem Methyl-CpG-binding domain protein 2 (0 Activities)

Discover Target: MBD2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000
IF/ICC	1/50-1/200 "> 1/50-1/200
IHC	1/50 - 1/100 "> 1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0506101	Certificate of Analysis	May 28, 2024	Ab114412
ZJ24F0506100	Certificate of Analysis	May 28, 2024	Ab114412

FAQs and Articles

FAQ

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: Protocols

Tags:

Frozen section Immunofluorescence staining Tissue section Methanol permeability IF

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
2. Hendrich, B B and Bird, A A.. (1998) Identification and characterization of a family of mammalian methyl-CpG binding proteins.. Molecular and cellular biology, [PMID:9774669]
3. Bhattacharya, S K SK, Ramchandani, S S, Cervoni, N N and Szyf, M M.. (1999) A mammalian protein with specific demethylase activity for mCpG DNA.. Nature, (18): [PMID:10050851]
4. Hendrich, B B and 5 more authors.. (1999) Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:10441743]
5. Ng, H H HH and 8 more authors.. (1999) MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex.. Nature genetics, [PMID:10471499]
6. Tatematsu, K I KI, Yamazaki, T T and Ishikawa, F F.. (2000) MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:10947852]
7. Boeke, J J, Ammerpohl, O O, Kegel, S S, Moehren, U U and Renkawitz, R R.. (2000) The minimal repression domain of MBD2b overlaps with the methyl-CpG-binding domain and binds directly to Sin3A.. The Journal of biological chemistry, (10): [PMID:10950960]
8. Humphrey, G W GW and 6 more authors.. (2001) Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.. The Journal of biological chemistry, (2): [PMID:11102443]
9. Sekimata, M M, Takahashi, A A, Murakami-Sekimata, A A and Homma, Y Y.. (2001) Involvement of a novel zinc finger protein, MIZF, in transcriptional repression by interacting with a methyl-CpG-binding protein, MBD2.. The Journal of biological chemistry, (16): [PMID:11553631]
10. Brackertz, Marc M, Boeke, Joern J, Zhang, Ru R and Renkawitz, Rainer R.. (2002) Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3.. The Journal of biological chemistry, (25): [PMID:12183469]
11. Lembo, Francesca F and 6 more authors.. (2003) MBDin, a novel MBD2-interacting protein, relieves MBD2 repression potential and reactivates transcription from methylated promoters.. Molecular and cellular biology, [PMID:12588985]
12. Fujita, Hidetoshi H and 5 more authors.. (2003) Antithetic effects of MBD2a on gene regulation.. Molecular and cellular biology, [PMID:12665568]
13. Jin, Seung-Gi SG, Jiang, Chun-Ling CL, Rauch, Tibor T, Li, Hongwei H and Pfeifer, Gerd P GP.. (2005) MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing.. The Journal of biological chemistry, (1): [PMID:15701600]
14. Brackertz, Marc M, Gong, Zihua Z, Leers, Jörg J and Renkawitz, Rainer R.. (2006) p66alpha and p66beta of the Mi-2/NuRD complex mediate MBD2 and histone interaction.. Nucleic acids research, [PMID:16415179]
15. Le Guezennec, Xavier X and 6 more authors.. (2006) MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties.. Molecular and cellular biology, [PMID:16428440]
16. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
17. Spruijt, Cornelia G CG and 6 more authors.. (2010) CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.. Molecular bioSystems, [PMID:20523938]
18. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
19. Cramer, Jason M JM and 5 more authors.. (2014) Probing the dynamic distribution of bound states for methylcytosine-binding domains on DNA.. The Journal of biological chemistry, (17): [PMID:24307175]
20. Spruijt, Cornelia G CG and 15 more authors.. (2016) ZMYND8 Co-localizes with NuRD on Target Genes and Regulates Poly(ADP-Ribose)-Dependent Recruitment of GATAD2A/NuRD to Sites of DNA Damage.. Cell reports, (11): [PMID:27732854]
21. Hoffmeister, Helen H and 12 more authors.. (2017) CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality.. Nucleic acids research, (13): [PMID:28977666]

Solution Calculators

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SKU	Size	Availability	Price
Ab114412-10μl	10μl	In stock	$89.90
Ab114412-50μl	50μl	In stock	$269.90
Ab114412-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$379.90
Ab114412-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90

Recombinant MBD2 Antibody - Primary antibody, specific to MBD2, Rabbit IgG