| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab114419-10μl | 10μl | In stock | $69.90 | |
| Ab114419-50μl | 50μl | In stock | $199.90 | |
| Ab114419-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab114419-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human MBD3 Antibody; WB; Unconjugated
| Product Name | Recombinant MBD3 Antibody - Primary antibody, specific to MBD3, Rabbit IgG |
|---|---|
| Synonyms | MBD3 | methyl-CpG binding domain protein 3 | methyl-CpG-binding domain protein 3 | Methyl-CpG-binding protein MBD3 | AI181826 antibody | AU019209 antibody | MBD 3 antibody | Mbd3 antibody | MBD3: methyl CpG binding domain protein 3 antibody | MBD3_HUMAN a |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.5 mg/mL |
| Host species | Rabbit |
| Specificity | MBD3 |
| Immunogen | A synthetic peptide derived from human MBD3 (AA 46-103) |
| Positive Control | WB: HeLa, HEK293, MCF7, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human MBD3 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant MBD3 Antibody (Ab114419) - Western Blot
All lanes: Recombinant MBD3 Antibody (Ab114419) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33, 34 kDa
Exposure time: 10.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505535 | Certificate of Analysis | May 14, 2024 | Ab114419 |
| ZJ24F0505534 | Certificate of Analysis | May 14, 2024 | Ab114419 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Hendrich, B B and Bird, A A.. (1998) Identification and characterization of a family of mammalian methyl-CpG binding proteins.. Molecular and cellular biology, [PMID:9774669] |
| 3. Tatematsu, K I KI, Yamazaki, T T and Ishikawa, F F.. (2000) MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:10947852] |
| 4. Humphrey, G W GW and 6 more authors.. (2001) Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.. The Journal of biological chemistry, (2): [PMID:11102443] |
| 5. Feng, Qin Q and 5 more authors.. (2002) Identification and functional characterization of the p66/p68 components of the MeCP1 complex.. Molecular and cellular biology, [PMID:11756549] |
| 6. Saito, Motoki M and Ishikawa, Fuyuki F.. (2002) The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2.. The Journal of biological chemistry, (20): [PMID:12124384] |
| 7. Brackertz, Marc M, Boeke, Joern J, Zhang, Ru R and Renkawitz, Rainer R.. (2002) Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3.. The Journal of biological chemistry, (25): [PMID:12183469] |
| 8. Fujita, Naoyuki N and 6 more authors.. (2004) MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation.. Cell, (1): [PMID:15454082] |
| 9. Jin, Seung-Gi SG, Jiang, Chun-Ling CL, Rauch, Tibor T, Li, Hongwei H and Pfeifer, Gerd P GP.. (2005) MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing.. The Journal of biological chemistry, (1): [PMID:15701600] |
| 10. Le Guezennec, Xavier X and 6 more authors.. (2006) MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties.. Molecular and cellular biology, [PMID:16428440] |
| 11. Morey, Lluis L and 9 more authors.. (2008) MBD3, a component of the NuRD complex, facilitates chromatin alteration and deposition of epigenetic marks.. Molecular and cellular biology, [PMID:18644863] |
| 12. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 13. Spruijt, Cornelia G CG and 6 more authors.. (2010) CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.. Molecular bioSystems, [PMID:20523938] |
| 14. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 15. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 16. Günther, Katharina K and 7 more authors.. (2013) Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences.. Nucleic acids research, (1): [PMID:23361464] |
| 17. Cramer, Jason M JM and 5 more authors.. (2014) Probing the dynamic distribution of bound states for methylcytosine-binding domains on DNA.. The Journal of biological chemistry, (17): [PMID:24307175] |
| 18. Shimbo, Takashi T and 7 more authors.. (2013) MBD3 localizes at promoters, gene bodies and enhancers of active genes.. PLoS genetics, [PMID:24385926] |
| 19. Spruijt, Cornelia G CG and 15 more authors.. (2016) ZMYND8 Co-localizes with NuRD on Target Genes and Regulates Poly(ADP-Ribose)-Dependent Recruitment of GATAD2A/NuRD to Sites of DNA Damage.. Cell reports, (11): [PMID:27732854] |
| 20. Hoffmeister, Helen H and 12 more authors.. (2017) CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality.. Nucleic acids research, (13): [PMID:28977666] |
