Recombinant; Rabbit anti Human MCM6 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab114562-10μl | 10μl | In stock | $69.90 | |
| Ab114562-50μl | 50μl | In stock | $209.90 | |
| Ab114562-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $509.90 | |
| Ab114562-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,819.90 |
Recombinant; Rabbit anti Human MCM6 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant MCM6 Antibody - Primary antibody, specific to MCM6, Rabbit IgG |
|---|---|
| Synonyms | EC 3.6.4.12 | p105MCM | DNA replication licensing factor MCM6 antibodyMCG40308 antibody | Mcm6 antibody | MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody | MCM6 minichromosome maintenance deficient 6 antibody | MCM6_HUMAN ant |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | MCM6 |
| Immunogen | Recombinant protein of human MCM6 (AA 676-821) |
| Positive Control | WB: Jurkat, HeLa, MCF7, U2OS, Hep G2, A431, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: Hela cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human MCM6 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant MCM6 Antibody (Ab114562) - Western Blot
All lanes: Recombinant MCM6 Antibody (Ab114562) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 97 kDa
Exposure time: 50.8 s
Recombinant MCM6 Antibody (Ab114562) - IF/ICC
Immunocytochemistry analysis of MCM6 (green) in HeLa using Recombinant MCM6 Antibody (Ab114562), and cells were counterstained with DAPI (blue).
Recombinant MCM6 Antibody (Ab114562) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant MCM6 Antibody (Ab114562). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303902 | Certificate of Analysis | Apr 02, 2024 | Ab114562 |
| ZJ24F0404053 | Certificate of Analysis | Apr 02, 2024 | Ab114562 |
| 1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
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| 5. Harvey, C B CB and 5 more authors.. (1996) Characterisation of a human homologue of a yeast cell division cycle gene, MCM6, located adjacent to the 5' end of the lactase gene on chromosome 2q21.. FEBS letters, (2): [PMID:8977093] |
| 6. Tsuruga, H H and 5 more authors.. (1997) HsMCM6: a new member of the human MCM/P1 family encodes a protein homologous to fission yeast Mis5.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:9286856] |
| 7. and Ishimi, Y Y.. (1997) A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.. The Journal of biological chemistry, (26): [PMID:9305914] |
| 8. Holthoff, H P HP, Baack, M M, Richter, A A, Ritzi, M M and Knippers, R R.. (1998) Human protein MCM6 on HeLa cell chromatin.. The Journal of biological chemistry, (27): [PMID:9516426] |
| 9. Izumi, M M and 8 more authors.. (2000) The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase.. Nucleic acids research, (1): [PMID:11095689] |
| 10. Enattah, Nabil Sabri NS and 5 more authors.. (2002) Identification of a variant associated with adult-type hypolactasia.. Nature genetics, [PMID:11788828] |
| 11. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 12. Tsuji, Toshiya T, Ficarro, Scott B SB and Jiang, Wei W.. (2006) Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells.. Molecular biology of the cell, [PMID:16899510] |
| 13. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 14. Chou, Danny M DM and Elledge, Stephen J SJ.. (2006) Tipin and Timeless form a mutually protective complex required for genotoxic stress resistance and checkpoint function.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17116885] |
| 15. Sakwe, Amos M AM, Nguyen, Tin T, Athanasopoulos, Vicki V, Shire, Kathy K and Frappier, Lori L.. (2007) Identification and characterization of a novel component of the human minichromosome maintenance complex.. Molecular and cellular biology, [PMID:17296731] |
| 16. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 17. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 18. Wei, Zhun Z and 6 more authors.. (2010) Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6.. The Journal of biological chemistry, (23): [PMID:20202939] |
| 19. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 20. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 21. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 22. Ishimi, Yukio Y and Irie, Daiki D.. (2015) G364R mutation of MCM4 detected in human skin cancer cells affects DNA helicase activity of MCM4/6/7 complex.. Journal of biochemistry, [PMID:25661590] |
| 23. Kottemann, Molly C MC, Conti, Brooke A BA, Lach, Francis P FP and Smogorzewska, Agata A.. (2018) Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.. Molecular cell, (4): [PMID:29290612] |
