A synthetic peptide derived from human MED4 (AA 150-250).
Positive Control
WB: Jurkat and HTP-1 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human MED4 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MED4 Antibody (Ab114775) - Western Blot All lanes: Recombinant MED4 Antibody (Ab114775) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 33 kDa Exposure time: 1.0 s
Recombinant MED4 Antibody (Ab114775) - Western Blot All lanes: Recombinant MED4 Antibody (Ab114775) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 33 kDa Exposure time: 4.1 s
Associated Targets(Human)
MED4TbioMediator of RNA polymerase II transcription subunit 4 (0 Activities)