Basic Description

Product Name	Recombinant MEF2C Antibody - Primary antibody, specific to MEF2C, Rabbit IgG
Synonyms	C5DELq14.3 \| NEDHSIL \| DEL5q14.3 \| MEF2C \| myocyte enhancer factor 2C \| myocyte-specific enhancer factor 2C \| polypeptide C \| C5DELq14.3 antibody \| DEL5q14.3 antibody \| MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) an
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species	Rabbit
Specificity	MEF2C
Immunogen	A synthetic peptide derived from human MEF2C (AA 400-445)
Positive Control	WB: Raji, K562 and C6 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human MEF2C Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant MEF2C Antibody (Ab114806) - Western Blot
All lanes: Recombinant MEF2C Antibody (Ab114806) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 51 kDa
Observed band size: 44-60 kDa
Exposure time: 8.7 s

Associated Targets(Human)

MEF2C Tbio Myocyte-specific enhancer factor 2C (0 Activities)

Discover Target: MEF2C

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0506105	Certificate of Analysis	May 28, 2024	Ab114806
ZJ24F0506104	Certificate of Analysis	May 28, 2024	Ab114806

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References

1. Han J, Jiang Y, Li Z, Kravchenko VV, Ulevitch RJ. (1997) Activation of the transcription factor MEF2C by the MAP kinase p38 in inflammation.. Nature, 386 (6622): (296-9). [PMID:9069290] [10.1021/op500134e]
2. Leifer, D D and 9 more authors.. (1993) MEF2C, a MADS/MEF2-family transcription factor expressed in a laminar distribution in cerebral cortex.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:7679508]
3. McDermott, J C JC and 7 more authors.. (1993) hMEF2C gene encodes skeletal muscle- and brain-specific transcription factors.. Molecular and cellular biology, [PMID:8455629]
4. Kato, Y Y and 5 more authors.. (1997) BMK1/ERK5 regulates serum-induced early gene expression through transcription factor MEF2C.. The EMBO journal, (1): [PMID:9384584]
5. Swanson, B J BJ, Jäck, H M HM and Lyons, G E GE.. (1998) Characterization of myocyte enhancer factor 2 (MEF2) expression in B and T cells: MEF2C is a B cell-restricted transcription factor in lymphocytes.. Molecular immunology, [PMID:9798649]
6. Wang, A H AH and 8 more authors.. (1999) HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor.. Molecular and cellular biology, [PMID:10523670]
7. Kato, Y Y and 10 more authors.. (2000) Big mitogen-activated kinase regulates multiple members of the MEF2 protein family.. The Journal of biological chemistry, (16): [PMID:10849446]
8. Zhou, X X, Marks, P A PA, Rifkind, R A RA and Richon, V M VM.. (2001) Cloning and characterization of a histone deacetylase, HDAC9.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:11535832]
9. Okamoto, Shu-ichi S and 8 more authors.. (2002) Dominant-interfering forms of MEF2 generated by caspase cleavage contribute to NMDA-induced neuronal apoptosis.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:11904443]
10. Zhu, Bangmin B and Gulick, Tod T.. (2004) Phosphorylation and alternative pre-mRNA splicing converge to regulate myocyte enhancer factor 2C activity.. Molecular and cellular biology, [PMID:15340086]
11. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022]
12. Gocke, Christian B CB, Yu, Hongtao H and Kang, Jungseog J.. (2005) Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates.. The Journal of biological chemistry, (11): [PMID:15561718]
13. Grégoire, Serge S and Yang, Xiang-Jiao XJ.. (2005) Association with class IIa histone deacetylases upregulates the sumoylation of MEF2 transcription factors.. Molecular and cellular biology, [PMID:15743823]
14. Ma, Kewei K, Chan, Jonathan K L JK, Zhu, Guang G and Wu, Zhenguo Z.. (2005) Myocyte enhancer factor 2 acetylation by p300 enhances its DNA binding activity, transcriptional activity, and myogenic differentiation.. Molecular and cellular biology, [PMID:15831463]
15. Zhu, Bangmin B, Ramachandran, Bindu B and Gulick, Tod T.. (2005) Alternative pre-mRNA splicing governs expression of a conserved acidic transactivation domain in myocyte enhancer factor 2 factors of striated muscle and brain.. The Journal of biological chemistry, (5): [PMID:15834131]
16. Kang, Jungseog J, Gocke, Christian B CB and Yu, Hongtao H.. (2006) Phosphorylation-facilitated sumoylation of MEF2C negatively regulates its transcriptional activity.. BMC biochemistry, (14): [PMID:16478538]
17. Le Meur, N N and 19 more authors.. (2010) MEF2C haploinsufficiency caused by either microdeletion of the 5q14.3 region or mutation is responsible for severe mental retardation with stereotypic movements, epilepsy and/or cerebral malformations.. Journal of medical genetics, [PMID:19592390]
18. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
19. Carvill, Gemma L GL and 33 more authors.. (2013) Targeted resequencing in epileptic encephalopathies identifies de novo mutations in CHD2 and SYNGAP1.. Nature genetics, [PMID:23708187]
20. Parrini, Elena E and 18 more authors.. (2017) Diagnostic Targeted Resequencing in 349 Patients with Drug-Resistant Pediatric Epilepsies Identifies Causative Mutations in 30 Different Genes.. Human mutation, [PMID:27864847]

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SKU	Size	Availability	Price
Ab114806-10μl	10μl	In stock	$69.90
Ab114806-50μl	50μl	In stock	$199.90
Ab114806-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$329.90
Ab114806-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

Recombinant MEF2C Antibody - Primary antibody, specific to MEF2C, Rabbit IgG