A synthetic peptide derived from human MEK2 (AA 1-50).
Positive Control
WB: A549, HeLa, Jurkat, A431, MCF7 cell lysates, Mouse brain, Mouse lung lysates; IHC: Human breast cancer; ICC/IF: HeLa
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human MEK2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
0.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MEK2 Antibody (Ab114868) - Western Blot All lanes: Recombinant MEK2 Antibody (Ab114868) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 0.8 s
Recombinant MEK2 Antibody (Ab114868) - Western Blot All lanes: Recombinant MEK2 Antibody (Ab114868) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 5.8 s
Recombinant MEK2 Antibody (Ab114868) - IF Immunocytochemistry analysis of MEK2 (green) in HeLa cells using Recombinant MEK2 Antibody (Ab114868) and cells were counterstained with DAPI (blue).
Recombinant MEK2 Antibody (Ab114868) – IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant MEK2 Antibody (Ab114868). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets(Human)
MAP2K2TclinDual specificity mitogen-activated protein kinase kinase 2 (0 Activities)