Recombinant Mesothelin Antibody - Primary antibody, specific to Mesothelin, Rabbit IgG
Synonyms
CAK 1 antibody; CAK1 antibody; CAK1 antigen antibody; cleaved form antibody; Megakaryocyte potentiating factorantibody; Mesothelin antibody; Mesothelin isoform 1 precursorantibody; MPF antibody; Msln antibody; MSLN_HUMAN antibody; Pre pro megakaryocyte po
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.8 mg/mL
Host species
Rabbit
Specificity
Mesothelin
Immunogen
A synthetic peptide derived from human Mesothelin (AA 550-570).
Positive Control
WB: HeLa lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Mesothelin Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.8 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Mesothelin Antibody (Ab115021) - Western Blot All lanes: Recombinant Mesothelin Antibody (Ab115021) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 69 kDa Observed band size: 44, 69 kDa Exposure time: 28.2 s
1.Kojima, T T and 6 more authors.. (1995) Molecular cloning and expression of megakaryocyte potentiating factor cDNA.. The Journal of biological chemistry, (15):[PMID:7665620]
2.Yamaguchi, N N and 5 more authors.. (1994) A novel cytokine exhibiting megakaryocyte potentiating activity from a human pancreatic tumor cell line HPC-Y5.. The Journal of biological chemistry, (14):[PMID:8288629]
3.Chang, K K and Pastan, I I.. (1996) Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers.. Proceedings of the National Academy of Sciences of the United States of America, (9):[PMID:8552591]
4.Scholler, N N and 6 more authors.. (1999) Soluble member(s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma.. Proceedings of the National Academy of Sciences of the United States of America, (28):[PMID:10500211]
5.Daniels, R J RJ and 10 more authors.. (2001) Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16.. Human molecular genetics, (15):[PMID:11157797]
6.Rump, Armin A and 6 more authors.. (2004) Binding of ovarian cancer antigen CA125/MUC16 to mesothelin mediates cell adhesion.. The Journal of biological chemistry, (5):[PMID:14676194]
7.Muminova, Zhanat E ZE, Strong, Theresa V TV and Shaw, Denise R DR.. (2004) Characterization of human mesothelin transcripts in ovarian and pancreatic cancer.. BMC cancer, (12):[PMID:15140265]
8.Ho, Mitchell M and 6 more authors.. (2005) Humoral immune response to mesothelin in mesothelioma and ovarian cancer patients.. Clinical cancer research : an official journal of the American Association for Cancer Research, (15):[PMID:15897581]
9.Onda, Masanori M and 6 more authors.. (2006) Megakaryocyte potentiation factor cleaved from mesothelin precursor is a useful tumor marker in the serum of patients with mesothelioma.. Clinical cancer research : an official journal of the American Association for Cancer Research, (15):[PMID:16857795]
10.Ma, Jichun J, Tang, Wai Kwan WK, Esser, Lothar L, Pastan, Ira I and Xia, Di D.. (2012) Recognition of mesothelin by the therapeutic antibody MORAb-009: structural and mechanistic insights.. The Journal of biological chemistry, (28):[PMID:22787150]
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