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Recombinant METTL3 Antibody - Primary antibody, specific to METTL3, Rabbit IgG

    Application:
  • WB
Features and benefitsView More
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab115170
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab115170-10μl
10μl
In stock
$66.90
Ab115170-50μl
50μl
In stock
$199.90
Ab115170-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab115170-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human METTL3 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant METTL3 Antibody - Primary antibody, specific to METTL3, Rabbit IgG
SynonymsIME4 | Methyltransferase like protein 3 | Methyltransferase-like protein 3 | Methyltransferase like 3 | METTL3 | MT-A70 | MTA70 | MTA70_HUMAN | N6 adenosine methyltransferase 70 kDa subunit | N6-adenosine-methyltransferase 70 kDa subunit | adoMet-binding
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificityMETTL3
ImmunogenA synthetic peptide derived from human METTL3 (AA 1-285)
Positive ControlWB: HEK293, Raji, HeLa, Hep G2, RAW 264.7 and C6 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human METTL3 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function
N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant METTL3 Antibody (Ab115170) - Western Blot
All lanes: Recombinant METTL3 Antibody (Ab115170) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 64 kDa
Observed band size: 71 kDa
Exposure time: 12.1 s

Recombinant METTL3 Antibody (Ab115170) - Western Blot
All lanes: Recombinant METTL3 Antibody (Ab115170) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 71 kDa
Exposure time: 0.3 s

Associated Targets(Human)

METTL3 Tbio N6-adenosine-methyltransferase catalytic subunit (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
METTL3 Tbio METTL3/METTL14 (156 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

Certificates

C of A & Other Certificates(BSE/TSE, COO)

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505539Certificate of AnalysisMay 14, 2024 Ab115170
ZJ24F0505538Certificate of AnalysisMay 14, 2024 Ab115170

Related Documents

References

1. Wang X, Feng J, Xue Y, Guan Z, Zhang D, Liu Z, Gong Z, Wang Q, Huang J, Tang C et al..  (2016)  Structural basis of N(6)-adenosine methylation by the METTL3-METTL14 complex..  Nature,  534  (7608): (575-8).  [PMID:27281194] [10.1021/op500134e]
2. Claudio R Alarcón,Hyeseung Lee,Hani Goodarzi,Nils Halberg,Sohail F Tavazoie.  (2015-03-25)  N6-methyladenosine marks primary microRNAs for processing..  Nature,  519  ((7544)): (482-485).  [PMID:25799998]
3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
4. Xiao Wang,Zhike Lu,Adrian Gomez,Gary C Hon,Yanan Yue,Dali Han,Ye Fu,Marc Parisien,Qing Dai,Guifang Jia,Bing Ren,Tao Pan,Chuan He.  (2013-11-29)  N6-methyladenosine-dependent regulation of messenger RNA stability..  Nature,  505  ((7481)): (117-120).  [PMID:24284625]
5. Shuibin Lin,Junho Choe,Peng Du,Robinson Triboulet,Richard I Gregory.  (2016-04-28)  The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells..  Molecular cell,  62  ((3)): (335-345).  [PMID:27117702]
6. Xiong Y, Li F, Babault N, Dong A, Zeng H, Wu H, Chen X, Arrowsmith CH, Brown PJ, Liu J, Vedadi M, Jin J..  (2017)  Discovery of Potent and Selective Inhibitors for G9a-Like Protein (GLP) Lysine Methyltransferase..  J Med Chem,  60  (5): (1876-1891).  [PMID:28135087] [10.1021/acs.jmedchem.6b01645]
7. Pan S,Zhou Y,Wang Q,Wang Y,Tian C,Wang T,Huang L,Nan J,Li L,Yang S.  (2020)  Discovery and structure-activity relationship studies of 1-aryl-1H-naphtho[2,3-d][1,2,3]triazole-4,9-dione derivatives as potent dual inhibitors of indoleamine 2,3-dioxygenase 1 (IDO1) and trytophan 2,3-dioxygenase (TDO)..  Eur J Med Chem,  207  (112703-112703).  [PMID:32871341] [10.1016/j.ejmech.2020.112703]
8. Bokar, J A JA, Shambaugh, M E ME, Polayes, D D, Matera, A G AG and Rottman, F M FM..  (1997)  Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase..  RNA (New York, N.Y.),      [PMID:9409616]
9. Heilig, Roland R and 98 more authors..  (2003)  The DNA sequence and analysis of human chromosome 14..  Nature,    (6):   [PMID:12508121]
10. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP..  (2006)  A probability-based approach for high-throughput protein phosphorylation analysis and site localization..  Nature biotechnology,      [PMID:16964243]
11. Matsuoka, Shuhei S and 12 more authors..  (2007)  ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage..  Science (New York, N.Y.),    (25):   [PMID:17525332]
12. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
13. Dominissini, Dan D and 11 more authors..  (2012)  Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq..  Nature,    (29):   [PMID:22575960]
14. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
15. Ping, Xiao-Li XL and 24 more authors..  (2014)  Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase..  Cell research,      [PMID:24407421]
16. Schwartz, Schraga S and 19 more authors..  (2014)  Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites..  Cell reports,    (10):   [PMID:24981863]
17. Liu, Nian N and 5 more authors..  (2015)  N(6)-methyladenosine-dependent RNA structural switches regulate RNA-protein interactions..  Nature,    (26):   [PMID:25719671]
18. Alarcón, Claudio R CR and 5 more authors..  (2015)  HNRNPA2B1 Is a Mediator of m(6)A-Dependent Nuclear RNA Processing Events..  Cell,    (10):   [PMID:26321680]
19. Zhou, Jun J and 5 more authors..  (2015)  Dynamic m(6)A mRNA methylation directs translational control of heat shock response..  Nature,    (22):   [PMID:26458103]
20. Meyer, Kate D KD and 8 more authors..  (2015)  5' UTR m(6)A Promotes Cap-Independent Translation..  Cell,    (5):   [PMID:26593424]
21. Wang, Ping P, Doxtader, Katelyn A KA and Nam, Yunsun Y..  (2016)  Structural Basis for Cooperative Function of Mettl3 and Mettl14 Methyltransferases..  Molecular cell,    (21):   [PMID:27373337]
22. Patil, Deepak P DP and 6 more authors..  (2016)  m(6)A RNA methylation promotes XIST-mediated transcriptional repression..  Nature,    (15):   [PMID:27602518]
23. Śledź, Paweł P and Jinek, Martin M..  (2016)  Structural insights into the molecular mechanism of the m(6)A writer complex..  eLife,    (14):   [PMID:27627798]
24. Xiang, Yang Y and 15 more authors..  (2017)  RNA m6A methylation regulates the ultraviolet-induced DNA damage response..  Nature,    (23):   [PMID:28297716]
25. Schöller, Eva E and 8 more authors..  (2018)  Interactions, localization, and phosphorylation of the m6A generating METTL3-METTL14-WTAP complex..  RNA (New York, N.Y.),      [PMID:29348140]
26. Du, Yuzhang Y and 16 more authors..  (2018)  SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function..  Nucleic acids research,    (1):   [PMID:29506078]
27. Yue, Yanan Y and 16 more authors..  (2018)  VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation..  Cell discovery,      [PMID:29507755]
28. Zhong, Xiang X and 17 more authors..  (2018)  Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m6A mRNA Methylation..  Cell reports,    (13):   [PMID:30428350]

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