ExactAb™, Validated, High performance, Recombinant, Lot by Lot
Host species
Rabbit
Specificity
MACROH2A1
Immunogen
A synthetic peptide derived from human macroH2A.1 (AA 220-275)
Positive Control
WB: HeLa, Hep G2, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Product Description
Rabbit anti Human mH2A1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/1000-1/5000 Protein Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
1.Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)):(4493-4501). [PMID:19413330]
2.Mao, M M and 16 more authors.. (1998) Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.. Proceedings of the National Academy of Sciences of the United States of America, (7):[PMID:9653160]
3.Behrends, Uta U and 17 more authors.. (2003) Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries.. International journal of cancer, (20):[PMID:12800201]
4.Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16):[PMID:15372022]
5.Zhang, Rugang R and 13 more authors.. (2005) Formation of MacroH2A-containing senescence-associated heterochromatin foci and senescence driven by ASF1a and HIRA.. Developmental cell, [PMID:15621527]
6.Hernández-Muñoz, Inmaculada I and 9 more authors.. (2005) Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase.. Proceedings of the National Academy of Sciences of the United States of America, (24):[PMID:15897469]
7.Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679]
8.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
9.Zhuang, Min M and 13 more authors.. (2009) Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.. Molecular cell, (9):[PMID:19818708]
10.Mehrotra, Pawan Vinod PV and 7 more authors.. (2011) DNA repair factor APLF is a histone chaperone.. Molecular cell, (7):[PMID:21211722]
11.Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15):[PMID:21406692]
12.Dardenne, Etienne E and 13 more authors.. (2012) Splicing switch of an epigenetic regulator by RNA helicases promotes tumor-cell invasiveness.. Nature structural & molecular biology, [PMID:23022728]
13.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
14.Rosenthal, Florian F and 11 more authors.. (2013) Macrodomain-containing proteins are new mono-ADP-ribosylhydrolases.. Nature structural & molecular biology, [PMID:23474714]
15.Corbeski, Ivan; Dolinar, Klemen; Wienk, Hans; Boelens, Rolf and van Ingen, Hugo.. (2018) DNA repair factor APLF acts as a H2A-H2B histone chaperone through binding its DNA interaction surface.. Nucleic acids research, (21):[PMID:29905837]
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