A synthetic peptide derived from human mtTFA (AA 57-70).
Positive Control
WB: A431, HEK293, A549, Hep G2 and HeLa cell ysates; IHC: Human breast cancer tissues; IF/ICC: HeLa.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human mtTFA Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Function Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant mtTFA Antibody (Ab116339) - Western Blot All lanes: Recombinant mtTFA Antibody (Ab116339) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 11.1 s
Recombinant mtTFA Antibody (Ab116339) - ICC/IF Immunocytochemistry analysis of beta Tubulin(green) in HeLa cells using Recombinant mtTFA Antibody (Ab116339) and cells were counterstained with DAPI (blue).
Recombinant mtTFA Antibody (Ab116339) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant mtTFA Antibody (Ab116339) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets(Human)
TFAMTbioTranscription factor A, mitochondrial (0 Activities)