Recombinant mtTFA Antibody - Primary antibody, specific to TFAM, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab116339
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab116339-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab116339-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab116339-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$297.90
Ab116339-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,217.90

Recombinant; Rabbit anti Human mtTFA Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant mtTFA Antibody - Primary antibody, specific to TFAM, Rabbit IgG
Synonymsanscription factor 6-like 1 antibody | MitochomtTFA ndrial transcription factor 1 antibody | mitochondrial transcription factor A antibody | MtTF1 antibody | mtTFA antibody | TCF 6 antibody | TCF-6 antibody | TCF6 antibody | TCF6L1 antibody | TCF6L2 antib
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificityTFAM
ImmunogenA synthetic peptide derived from human mtTFA (AA 57-70).
Positive ControlWB: A431, HEK293, A549, Hep G2 and HeLa cell ysates; IHC: Human breast cancer tissues; IF/ICC: HeLa.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human mtTFA Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant mtTFA Antibody (Ab116339) - Western Blot
All lanes: Recombinant mtTFA Antibody (Ab116339) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 11.1 s

Recombinant mtTFA Antibody (Ab116339) - ICC/IF
Immunocytochemistry analysis of beta Tubulin(green) in HeLa cells using Recombinant mtTFA Antibody (Ab116339) and cells were counterstained with DAPI (blue).

Recombinant mtTFA Antibody (Ab116339) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant mtTFA Antibody (Ab116339) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

TFAM Tbio Transcription factor A, mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

IF/ICC1/50 - 1/200

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1/50 - 1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800831Certificate of AnalysisAug 16, 2023 Ab116339
ZJ23F0800830Certificate of AnalysisAug 16, 2023 Ab116339

Related Documents

Solution Calculators