A synthetic peptide derived from human MUC4 (AA 1564-1673).
Positive Control
WB: MCF-7, A549 and HeLa cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human MUC4 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 – 1/1000 Protein Function May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.65 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MUC4 Antibody (Ab116451) - Western Blot All lanes: Recombinant MUC4 Antibody (Ab116451) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 120 kDa Observed band size: 118 kDa Exposure time: 23.9 s
1.Porchet, N N and 9 more authors.. (1991) Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs.. Biochemical and biophysical research communications, (15):[PMID:1673336]
2.Nollet, S S and 7 more authors.. (1998) Human mucin gene MUC4: organization of its 5'-region and polymorphism of its central tandem repeat array.. The Biochemical journal, (15):[PMID:9620877]
3.Moniaux, N N and 5 more authors.. (1999) Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin.. The Biochemical journal, (1):[PMID:10024507]
4.Moniaux, N N and 5 more authors.. (2000) Alternative splicing generates a family of putative secreted and membrane-associated MUC4 mucins.. European journal of biochemistry, [PMID:10880978]
5.Choudhury, A A and 5 more authors.. (2000) Human MUC4 mucin cDNA and its variants in pancreatic carcinoma.. Journal of biochemistry, [PMID:10920259]
6.Desseyn, Jean-Luc JL, Clavereau, Isabelle I and Laine, Anne A.. (2002) Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4.. European journal of biochemistry, [PMID:12084055]
7.Carraway, Kermit L KL and 9 more authors.. (2002) Muc4/sialomucin complex, the intramembrane ErbB2 ligand, in cancer and epithelia: to protect and to survive.. Progress in nucleic acid research and molecular biology, [PMID:12102554]
8.Escande, Fabienne F and 5 more authors.. (2002) Genomic organization of MUC4 mucin gene. Towards the characterization of splice variants.. European journal of biochemistry, [PMID:12153560]
9.Saitou, M M and 11 more authors.. (2005) MUC4 expression is a novel prognostic factor in patients with invasive ductal carcinoma of the pancreas.. Journal of clinical pathology, [PMID:16049287]
10.Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27):[PMID:16641997]
11.Karg, Aydanur A, Dinç, Zekiye Aydoğdu ZA, Başok, Oktay O and Uçvet, Ahmet A.. (2006) MUC4 expression and its relation to ErbB2 expression, apoptosis, proliferation, differentiation, and tumor stage in non-small cell lung cancer (NSCLC).. Pathology, research and practice, [PMID:16814944]
12.Zhang, Jin J, Yasin, Mohammad M, Carraway, Coralie A Carothers CA and Carraway, Kermit L KL.. (2006) MUC4 expression and localization in gastrointestinal tract and skin of human embryos.. Tissue & cell, [PMID:16914178]
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