Recombinant NCE2/UBE2F Antibody - Primary antibody, specific to UBE2F, Rabbit IgG

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab117225
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab117225-10μl
10μl
In stock
$89.90
Ab117225-50μl
50μl
In stock
$269.90
Ab117225-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab117225-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human NCE2/UBE2F Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant NCE2/UBE2F Antibody - Primary antibody, specific to UBE2F, Rabbit IgG
SynonymsNCE2 | NEDD8 carrier protein UBE2F | NEDD8 conjugating enzyme | NEDD8 conjugating enzyme UBE2F | NEDD8 protein ligase UBE2F | NEDD8-conjugating enzyme 2 | NEDD8-conjugating enzyme UBE2F | ube2f | UBE2F_HUMAN | Ubiquitin conjugating enzyme E2F | Ubiquitin-
Specifications & PurityExactAb™, Validated, Recombinant, 0.9 mg/mL
Host speciesRabbit
SpecificityUBE2F
ImmunogenRecombinant protein of human NCE2 (AA 9-114)
Positive ControlWB: HeLa, MCF7, Jurkat and A375 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human NCE2/UBE2F Antibody, Recombinant, could be used for WB and so on.

Application:
WB: 1/500-1/1000

Protein Function
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.9 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant NCE2/UBE2F Antibody (Ab117225) - Western Blot
All lanes: Recombinant NCE2/UBE2F Antibody (Ab117225) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 17, 18, 22 kDa
Exposure time: 30.0 s

Associated Targets(Human)

UBE2F Tbio NEDD8-conjugating enzyme UBE2F (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506123Certificate of AnalysisMay 27, 2024 Ab117225
ZJ24F0506122Certificate of AnalysisMay 27, 2024 Ab117225

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