| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab117357-10μl | 10μl | In stock | $89.90 | |
| Ab117357-50μl | 50μl | In stock | $269.90 | |
| Ab117357-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $379.90 | |
| Ab117357-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human NDUFAF5 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant NDUFAF5 Antibody - Primary antibody, specific to NDUFAF5, Rabbit IgG |
|---|---|
| Synonyms | 2.1.1.- | EC 1.-.-.- | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 | Putative methyltransferase NDUFAF5 | C20orf7 | chromosome 20 open reading frame 7 | CT007_HUMAN | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly fac |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 2.0 mg/mL |
| Host species | Rabbit |
| Specificity | NDUFAF5 |
| Immunogen | Recombinant protein of human NDUFAF5 (AA 205-345) |
| Positive Control | WB: Hep G2, Jurkat, MCF7, HeLa and NIH/3T3 cell lysates. IHC: Human breast cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human NDUFAF5 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 2.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant NDUFAF5 Antibody (Ab117357) - Western Blot
All lanes: Recombinant NDUFAF5 Antibody (Ab117357) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 37 kDa
Exposure time: 23.1 s
Recombinant NDUFAF5 Antibody (Ab117357) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant NDUFAF5 Antibody (Ab117357). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506131 | Certificate of Analysis | May 27, 2024 | Ab117357 |
| ZJ24F0506130 | Certificate of Analysis | May 27, 2024 | Ab117357 |
| 1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] [10.1021/op500134e] |
| 2. Sugiana, Canny C and 15 more authors.. (2008) Mutation of C20orf7 disrupts complex I assembly and causes lethal neonatal mitochondrial disease.. American journal of human genetics, [PMID:18940309] |
| 3. Gerards, M M and 12 more authors.. (2010) Defective complex I assembly due to C20orf7 mutations as a new cause of Leigh syndrome.. Journal of medical genetics, [PMID:19542079] |
| 4. Saada, Ann A and 7 more authors.. (2012) Combined OXPHOS complex I and IV defect, due to mutated complex I assembly factor C20ORF7.. Journal of inherited metabolic disease, [PMID:21607760] |
| 5. Rhein, Virginie F VF, Carroll, Joe J, Ding, Shujing S, Fearnley, Ian M IM and Walker, John E JE.. (2016) NDUFAF5 Hydroxylates NDUFS7 at an Early Stage in the Assembly of Human Complex I.. The Journal of biological chemistry, (8): [PMID:27226634] |
| 6. Floyd, Brendan J BJ and 23 more authors.. (2016) Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.. Molecular cell, (18): [PMID:27499296] |
