Recombinant; Rabbit anti Human NEDD4 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab117482-10μl | 10μl | In stock | $69.90 | |
| Ab117482-50μl | 50μl | In stock | $199.90 | |
| Ab117482-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab117482-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human NEDD4 Antibody; WB; Unconjugated
| Product Name | Recombinant NEDD4 Antibody - Primary antibody, specific to NEDD4, Rabbit IgG |
|---|---|
| Synonyms | NEDD4-1 | Cell proliferation-inducing gene 53 protein | E3 ubiquitin-protein ligase NEDD4 | EC 6.3.2 | EC 6.3.2.- | KIAA0093NEDD4-1MGC176705 | NEDD4 | NEDD-4 | Neural precursor cell expressed developmentally down-regulated protein 4 | neural precursor cel |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | NEDD4 |
| Immunogen | A synthetic peptide derived from human NEDD4 (AA 1286-1306) |
| Positive Control | WB: HeLa, A549, U2OS, K562, MCF7, LNCaP, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human NEDD4 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant NEDD4 Antibody (Ab117482) - Western Blot
All lanes: Recombinant NEDD4 Antibody (Ab117482) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 113,128 kDa
Exposure time: 3.9 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303454 | Certificate of Analysis | Mar 20, 2024 | Ab117482 |
| ZJ24F0303453 | Certificate of Analysis | Mar 20, 2024 | Ab117482 |
| 1. Nagase, T T and 9 more authors.. (1995) Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:7788527] |
| 2. Hamilton, M H MH, Tcherepanova, I I, Huibregtse, J M JM and McDonnell, D P DP.. (2001) Nuclear import/export of hRPF1/Nedd4 regulates the ubiquitin-dependent degradation of its nuclear substrates.. The Journal of biological chemistry, (13): [PMID:11342538] |
| 3. Pham, N N and Rotin, D D.. (2001) Nedd4 regulates ubiquitination and stability of the guanine-nucleotide exchange factor CNrasGEF.. The Journal of biological chemistry, (14): [PMID:11598133] |
| 4. Timmins, Joanna J and 6 more authors.. (2003) Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4.. Journal of molecular biology, (14): [PMID:12559917] |
| 5. Bouamr, Fadila F and 6 more authors.. (2003) PPPYVEPTAP motif is the late domain of human T-cell leukemia virus type 1 Gag and mediates its functional interaction with cellular proteins Nedd4 and Tsg101 [corrected].. Journal of virology, [PMID:14581525] |
| 6. Zody, Michael C MC and 68 more authors.. (2006) Analysis of the DNA sequence and duplication history of human chromosome 15.. Nature, (30): [PMID:16572171] |
| 7. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 8. Wang, Xinjiang X and 11 more authors.. (2007) NEDD4-1 is a proto-oncogenic ubiquitin ligase for PTEN.. Cell, (12): [PMID:17218260] |
| 9. Okumura, Atsushi A, Pitha, Paula M PM and Harty, Ronald N RN.. (2008) ISG15 inhibits Ebola VP40 VLP budding in an L-domain-dependent manner by blocking Nedd4 ligase activity.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:18305167] |
| 10. Ushijima, Yoko Y, Koshizuka, Tetsuo T, Goshima, Fumi F, Kimura, Hiroshi H and Nishiyama, Yukihiro Y.. (2008) Herpes simplex virus type 2 UL56 interacts with the ubiquitin ligase Nedd4 and increases its ubiquitination.. Journal of virology, [PMID:18353951] |
| 11. Putz, Ulrich U and 6 more authors.. (2008) Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins.. The Journal of biological chemistry, (21): [PMID:18819914] |
| 12. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 13. Harreman, Michelle M and 10 more authors.. (2009) Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation.. Proceedings of the National Academy of Sciences of the United States of America, (8): [PMID:19920177] |
| 14. Lin, Qiong Q and 5 more authors.. (2010) HECT E3 ubiquitin ligase Nedd4-1 ubiquitinates ACK and regulates epidermal growth factor (EGF)-induced degradation of EGF receptor and ACK.. Molecular and cellular biology, [PMID:20086093] |
| 15. Kawabe, Hiroshi H and 16 more authors.. (2010) Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development.. Neuron, (11): [PMID:20159449] |
| 16. Nabhan, Joseph F JF, Pan, Hui H and Lu, Quan Q.. (2010) Arrestin domain-containing protein 3 recruits the NEDD4 E3 ligase to mediate ubiquitination of the beta2-adrenergic receptor.. EMBO reports, [PMID:20559325] |
| 17. Rauch, Susanne S and Martin-Serrano, Juan J.. (2011) Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding.. Journal of virology, [PMID:21191027] |
| 18. Persaud, Avinash A and 8 more authors.. (2011) Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function.. The EMBO journal, (15): [PMID:21765395] |
| 19. Milkereit, Ruth R and Rotin, Daniela D.. (2011) A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.. PloS one, [PMID:22096579] |
| 20. Pareja, F F and 18 more authors.. (2012) Deubiquitination of EGFR by Cezanne-1 contributes to cancer progression.. Oncogene, (25): [PMID:22179831] |
| 21. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 22. Han, Sang-oh SO and 7 more authors.. (2012) MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound β(2)-adrenergic receptors.. The Journal of cell biology, (26): [PMID:23166351] |
| 23. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 24. Maspero, Elena E and 6 more authors.. (2013) Structure of a ubiquitin-loaded HECT ligase reveals the molecular basis for catalytic priming.. Nature structural & molecular biology, [PMID:23644597] |
| 25. Yazicioglu, Mustafa N MN and 7 more authors.. (2013) Cellular localization and characterization of cytosolic binding partners for Gla domain-containing proteins PRRG4 and PRRG2.. The Journal of biological chemistry, (6): [PMID:23873930] |
| 26. Qi, Shiqian S, O'Hayre, Morgan M, Gutkind, J Silvio JS and Hurley, James H JH.. (2014) Structural and biochemical basis for ubiquitin ligase recruitment by arrestin-related domain-containing protein-3 (ARRDC3).. The Journal of biological chemistry, (21): [PMID:24379409] |
| 27. Low, Ley-Hian LH and 8 more authors.. (2015) Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.. The Journal of biological chemistry, (13): [PMID:25631046] |
| 28. Noyes, Nathaniel C NC, Hampton, Brian B, Migliorini, Mary M and Strickland, Dudley K DK.. (2016) Regulation of Itch and Nedd4 E3 Ligase Activity and Degradation by LRAD3.. Biochemistry, (1): [PMID:26854353] |
