Recombinant NELFe Antibody - Primary antibody, specific to NELFE, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab117537
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab117537-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab117537-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$269.90
Ab117537-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab117537-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human NELFe Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant NELFe Antibody - Primary antibody, specific to NELFE, Rabbit IgG
SynonymsMajor histocompatibility complex gene RD | Negative elongation factor E | Negative elongation factor polypeptide E | NELF e | NELF-E | Nelfe | NELFE_HUMAN | Nuclear protein | RD | RD protein | RDBP | RD RNA binding protein | RDP | RNA-binding protein RD |
Specifications & PurityExactAb™, Validated, Recombinant, 0.3 mg/mL
Host speciesRabbit
SpecificityNELFE
ImmunogenRecombinant protein of human NELFe (AA 1-150)
Positive ControlWB: HeLa and NIH/3T3 cell lysates. IHC: Human breast cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human NELFe Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
Protein Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant NELFe Antibody (Ab117537) - Western Blot
All lanes: Recombinant NELFe Antibody (Ab117537) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 45, 47 kDa
Exposure time: 2.3 s 

Recombinant NELFe Antibody (Ab117537) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant NELFe Antibody (Ab117537). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

GBP1 Tbio Guanylate-binding protein 1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
NELFE Tbio Negative elongation factor E (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606436Certificate of AnalysisJun 03, 2024 Ab117537
ZJ24F0606435Certificate of AnalysisJun 03, 2024 Ab117537

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