A synthetic peptide derived from human Nicastrin (AA 696-709).
Positive Control
WB: HeLa, K562, Jurkat, Hep G2 and NIH/3T3 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Nicastrin Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Nicastrin Antibody (Ab118034) - Western Blot All lanes: Recombinant Nicastrin Antibody (Ab118034) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 78 kDa Observed band size: 110-125 kDa Exposure time: 52.9 s
Recombinant Nicastrin Antibody (Ab118034) - Western Blot All lanes: Recombinant Nicastrin Antibody (Ab118034) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 78 kDa Observed band size: 110-125 kDa Exposure time: 52.9 s