Recombinant NR2F2 Antibody - Primary antibody, specific to NR2F2, Rabbit IgG
Synonyms
Apolipoprotein A-I regulatory protein 1 antibody; Apolipoprotein AI regulatory protein 1 antibody; ARP-1 antibody; ARP1 antibody; COT2_HUMAN antibody; COUP TF2 antibody; COUP transcription factor 2 antibody; COUP transcription factor II antibody; COUP-TF
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.8 mg/mL
Host species
Rabbit
Specificity
NR2F2
Immunogen
A synthetic peptide derived from human NR2F2 (AA 1-32)
Positive Control
WB: MCF7, HEK293, K562 and NIH/3T3 cell lysates. IHC: Human breast cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human NR2F2 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500 - 1/1000 IHC: 1/50-1/100 Protein Function Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.8 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant NR2F2 Antibody (Ab118688) - Western Blot All lanes: Recombinant NR2F2 Antibody (Ab118688) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 46 kDa Observed band size: 42 kDa Exposure time: 74.2 s
Recombinant NR2F2 Antibody (Ab118688) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant NR2F2 Antibody (Ab118688). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
1.Ladias JA, Karathanasis SK. (1991) Regulation of the apolipoprotein AI gene by ARP-1, a novel member of the steroid receptor superfamily.. Science, 251 (4993):(561-5). [PMID:1899293]
2.Avram D, Ishmael JE, Nevrivy DJ, Peterson VJ, Lee SH, Dowell P, Leid M. (1999) Heterodimeric interactions between chicken ovalbumin upstream promoter-transcription factor family members ARP1 and ear2.. J Biol Chem, 274 (20):(14331-6). [PMID:10318855]
3.Kruse SW, Suino-Powell K, Zhou XE, Kretschman JE, Reynolds R, Vonrhein C, Xu Y, Wang L, Tsai SY, Tsai MJ, Xu HE. (2008) Identification of COUP-TFII orphan nuclear receptor as a retinoic acid-activated receptor.. PLoS Biol, 6 (9):(e227). [PMID:18798693]
4.Solt LA, Wang Y, Banerjee S, Hughes T, Kojetin DJ, Lundasen T, Shin Y, Liu J, Cameron MD, Noel R et al.. (2012) Regulation of circadian behaviour and metabolism by synthetic REV-ERB agonists.. Nature, 485 (7396):(62-8). [PMID:22460951]
5.Wang, L H LH, Ing, N H NH, Tsai, S Y SY, O'Malley, B W BW and Tsai, M J MJ.. (1991) The COUP-TFs compose a family of functionally related transcription factors.. Gene expression, [PMID:1820218]
6.Chu, K K and Zingg, H H HH.. (1997) The nuclear orphan receptors COUP-TFII and Ear-2 act as silencers of the human oxytocin gene promoter.. Journal of molecular endocrinology, [PMID:9343308]
7.Zody, Michael C MC and 68 more authors.. (2006) Analysis of the DNA sequence and duplication history of human chromosome 15.. Nature, (30):[PMID:16572171]
8.Al Turki, Saeed S and 40 more authors.. (2014) Rare variants in NR2F2 cause congenital heart defects in humans.. American journal of human genetics, (3):[PMID:24702954]
9.Bashamboo, Anu A and 17 more authors.. (2018) Loss of Function of the Nuclear Receptor NR2F2, Encoding COUP-TF2, Causes Testis Development and Cardiac Defects in 46,XX Children.. American journal of human genetics, (1):[PMID:29478779]
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