| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab118720-10μl | 10μl | In stock | $69.90 | |
| Ab118720-50μl | 50μl | In stock | $199.90 | |
| Ab118720-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab118720-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human NRF1 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant NRF1 Antibody - Primary antibody, specific to NRF1, Rabbit IgG | |
|---|---|---|
| Synonyms | alpha pal antibody | alpha palindromic binding protein antibody | Alpha palindromic-binding protein antibody | Alpha-pal antibody | locus control region factor 1 antibody | NFE2 related factor 1 antibody | NRF-1 antibody | Nrf1 antibody | NRF1_HUMAN antib | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL | |
| Host species | Rabbit | |
| Specificity | NRF1 | |
| Immunogen | A synthetic peptide derived from human NRF1 (AA 354-367) | |
| Positive Control | WB: K562, Hep G2, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells. | |
| Conjugation | Unconjugated | |
| Grade | ExactAb™, Recombinant, Validated | |
| Product Description | Rabbit anti Human NRF1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application:
Protein Function Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Post-translational Phosphorylation enhances DNA binding. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant NRF1 Antibody (Ab118720) - Western Blot
All lanes: Recombinant NRF1 Antibody (Ab118720) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 57, 64 kDa
Exposure time: 61.0 s
Recombinant NRF1 Antibody (Ab118720) - IF/ICC
Immunocytochemistry analysis of NRF1 (green) in HeLa using Recombinant NRF1 Antibody (Ab118720), and cells were counterstained with DAPI (blue).
Recombinant NRF1 Antibody (Ab118720) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant NRF1 Antibody (Ab118720). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
| IF/ICC | 1/50-1/200 "> 1/50-1/200 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303462 | Certificate of Analysis | Mar 20, 2024 | Ab118720 |
| ZJ24F0303461 | Certificate of Analysis | Mar 20, 2024 | Ab118720 |
| 1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205] [10.1021/op500134e] |
| 2. Gopalakrishnan, L L and Scarpulla, R C RC.. (1995) Structure, expression, and chromosomal assignment of the human gene encoding nuclear respiratory factor 1.. The Journal of biological chemistry, (28): [PMID:7629110] |
| 3. Efiok, B J BJ, Chiorini, J A JA and Safer, B B.. (1994) A key transcription factor for eukaryotic initiation factor-2 alpha is strongly homologous to developmental transcription factors and may link metabolic genes to cellular growth and development.. The Journal of biological chemistry, (22): [PMID:8034649] |
| 4. Virbasius, C A CA, Virbasius, J V JV and Scarpulla, R C RC.. (1993) NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of developmental regulators.. Genes & development, [PMID:8253388] |
| 5. Spelbrink, J N JN and Van den Bogert, C C.. (1995) The pre-mRNA of nuclear respiratory factor 1, a regulator of mitochondrial biogenesis, is alternatively spliced in human tissues and cell lines.. Human molecular genetics, [PMID:8541844] |
| 6. Gugneja, S S and Scarpulla, R C RC.. (1997) Serine phosphorylation within a concise amino-terminal domain in nuclear respiratory factor 1 enhances DNA binding.. The Journal of biological chemistry, (25): [PMID:9228045] |
| 7. Andersson, U U and Scarpulla, R C RC.. (2001) Pgc-1-related coactivator, a novel, serum-inducible coactivator of nuclear respiratory factor 1-dependent transcription in mammalian cells.. Molecular and cellular biology, [PMID:11340167] |
| 8. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
