Recombinant OTUB1 Antibody - Primary antibody, specific to OTUB1, Rabbit IgG
Synonyms
Deubiquitinating enzyme OTUB1 antibody | hOTU1 antibody | HSPC263 antibody | OTB1 antibody | OTU domain containing ubiquitin aldehyde binding protein 1 antibody | OTU domain, ubiquitin aldehyde binding 1 antibody | OTU domain-containing Ubal-binding prote
Specifications & Purity
ExactAb™, Validated, Recombinant, 1.3 mg/mL
Host species
Rabbit
Specificity
OTUB1
Immunogen
A synthetic peptide derived from human OTUB1 (AA 248-260)
Positive Control
WB: K562, HeLa, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human OTUB1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 - 1/1000 Protein Function Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant OTUB1 Antibody (Ab119474) - Western Blot All lanes: Recombinant OTUB1 Antibody (Ab119474) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 32 kDa Exposure time: 19.6 s
Associated Targets(Human)
ZNF280CTdarkZinc finger protein 280C (0 Activities)