Recombinant OTUB1 Antibody - Primary antibody, specific to OTUB1, Rabbit IgG

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab119474
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab119474-10μl
10μl
In stock
$69.90
Ab119474-50μl
50μl
In stock
$199.90
Ab119474-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab119474-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human OTUB1 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant OTUB1 Antibody - Primary antibody, specific to OTUB1, Rabbit IgG
SynonymsDeubiquitinating enzyme OTUB1 antibody | hOTU1 antibody | HSPC263 antibody | OTB1 antibody | OTU domain containing ubiquitin aldehyde binding protein 1 antibody | OTU domain, ubiquitin aldehyde binding 1 antibody | OTU domain-containing Ubal-binding prote
Specifications & PurityExactAb™, Validated, Recombinant, 1.3 mg/mL
Host speciesRabbit
SpecificityOTUB1
ImmunogenA synthetic peptide derived from human OTUB1 (AA 248-260)
Positive ControlWB: K562, HeLa, NIH/3T3 and C6 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human OTUB1 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500 - 1/1000
Protein Function
Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration1.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant OTUB1 Antibody (Ab119474) - Western Blot
All lanes: Recombinant OTUB1 Antibody (Ab119474) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 32 kDa
Exposure time: 19.6 s

Associated Targets(Human)

ZNF280C Tdark Zinc finger protein 280C (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
OTUB1 Tbio Ubiquitin thioesterase OTUB1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506145Certificate of AnalysisMay 27, 2024 Ab119474
ZJ24F0506144Certificate of AnalysisMay 27, 2024 Ab119474

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