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Recombinant PABPN1 Antibody - Primary antibody, for WB,IHC various applications, from Human, generated in Rabbit, specific to Human PABPN1, Rabbit IgG

Recombinant

Validated

ExactAb™

0.5 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human PABPN1 Antibody; WB, IHC; Unconjugated
Application: WB,IHC
Species reactivity(Reacts with): Human,Mouse
Isotype: Rabbit IgG

Application:

Item Number

Ab119979

Grouped product items
SKU	Size	Availability	Price
Ab119979-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab119979-50μl	50μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$207.90
Ab119979-100μl	100μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$297.90
Ab119979-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,007.90

Recombinant; Rabbit anti Human PABPN1 Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: PABPN1

Accession#:Q86U42 Gene ID:8106 PABPN1

Basic Description

Product Name	Recombinant PABPN1 Antibody - Primary antibody, for WB,IHC various applications, from Human, generated in Rabbit, specific to Human PABPN1, Rabbit IgG
Synonyms	Nuclear poly(A)-binding protein 1 antibody; OPMD antibody; PAB2 antibody; PABII antibody; PABP 2 antibody; pABP-2 antibody; PABP2 antibody; PABP2_HUMAN antibody; PABPII antibody; Pabpn1 antibody; poly(A) binding protein nuclear 1 antibody; Poly(A)-binding
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host Species	Rabbit
Host species	Rabbit
Specificity	Human PABPN1
Application	WB,IHC
Immunogen	A synthetic peptide derived from human PABPN1 (AA 16-28).
Positive Control	WB: HeLa, Raji, MCF7, Hep G2, Jurkat lysates. IHC: Human breast cancer tissue .
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human PABPN1 Antibody, Recombinant, could be used for WB and IHC. Application： WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.

Product Properties

Antibody Type	Primary antibody
Clonality	Recombinant
Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Source	Cell supernatant
Form	Liquid
Concentration	0.5 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Application

Dilution info

1/500-1/1000

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0700657	Certificate of Analysis	Jul 21, 2023	Ab119979
ZJ23F0700658	Certificate of Analysis	Jul 21, 2023	Ab119979

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
2. Brais, B B and 18 more authors.. (1998) Short GCG expansions in the PABP2 gene cause oculopharyngeal muscular dystrophy.. Nature genetics, [PMID:9462747]
3. Chen, Z Z, Li, Y Y and Krug, R M RM.. (1999) Influenza A virus NS1 protein targets poly(A)-binding protein II of the cellular 3'-end processing machinery.. The EMBO journal, (15): [PMID:10205180]
4. Calado, A A, Kutay, U U, Kühn, U U, Wahle, E E and Carmo-Fonseca, M M.. (2000) Deciphering the cellular pathway for transport of poly(A)-binding protein II.. RNA (New York, N.Y.), [PMID:10688363]
5. Calado, A A and 6 more authors.. (2000) Nuclear inclusions in oculopharyngeal muscular dystrophy consist of poly(A) binding protein 2 aggregates which sequester poly(A) RNA.. Human molecular genetics, (22): [PMID:11001936]
6. Kim, Y J YJ and 5 more authors.. (2001) The product of an oculopharyngeal muscular dystrophy gene, poly(A)-binding protein 2, interacts with SKIP and stimulates muscle-specific gene expression.. Human molecular genetics, (15): [PMID:11371506]
7. Fan, X X, Dion, P P, Laganiere, J J, Brais, B B and Rouleau, G A GA.. (2001) Oligomerization of polyalanine expanded PABPN1 facilitates nuclear protein aggregation that is associated with cell death.. Human molecular genetics, (1): [PMID:11689481]
8. van der Sluijs, Barbara M BM, van Engelen, Baziel G M BG and Hoefsloot, Lies H LH.. (2003) Oculopharyngeal muscular dystrophy (OPMD) due to a small duplication in the PABPN1 gene.. Human mutation, [PMID:12673802]
9. Sugaya, Keizo K, Matsubara, Shiro S, Miyamoto, Kazuhito K, Kawata, Akihiro A and Hayashi, Hideaki H.. (2003) An aggregate-prone conformational epitope in trinucleotide repeat diseases.. Neuroreport, (19): [PMID:14663186]
10. Dettwiler, Sabine S, Aringhieri, Chiara C, Cardinale, Stefano S, Keller, Walter W and Barabino, Silvia M L SM.. (2004) Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization.. The Journal of biological chemistry, (20): [PMID:15169763]
11. van Koningsbruggen, Silvana S and 6 more authors.. (2007) FRG1P-mediated aggregation of proteins involved in pre-mRNA processing.. Chromosoma, [PMID:17103222]
12. Jønson, Lars L and 8 more authors.. (2007) Molecular composition of IMP1 ribonucleoprotein granules.. Molecular & cellular proteomics : MCP, [PMID:17289661]
13. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
14. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
15. Skourti-Stathaki, Konstantina K, Proudfoot, Nicholas J NJ and Gromak, Natalia N.. (2011) Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination.. Molecular cell, (24): [PMID:21700224]
16. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
17. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
18. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
19. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
20. Matsubara, Shiro S and 5 more authors.. (2016) Nuclear inclusions mimicking poly(A)-binding protein nuclear 1 inclusions in a case of inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia with a novel mutation in the valosin-containing protein gene.. Neuromuscular disorders : NMD, [PMID:27209344]
21. Meola, Nicola N and 10 more authors.. (2016) Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts.. Molecular cell, (3): [PMID:27871484]

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