Recombinant; Rabbit anti Human PACT (PKR activating protein) / PRKRA Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab120014-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab120014-50μl | 50μl | In stock | $199.90 | |
| Ab120014-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab120014-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human PACT (PKR activating protein) / PRKRA Antibody; WB; Unconjugated
| Product Name | Recombinant PACT/PRKRA Antibody - Primary antibody, specific to PRKRA, Rabbit IgG |
|---|---|
| Synonyms | Recombinant PACT (PKR activating protein) / PRKRA Antibody 8、英文别名：DYT16; DYT16interferon-inducible double stranded RNA-dependent activator; HSD14; PACT; PACTPKR-associating protein X; PKR-associated protein X; PKR-Associating Protein X; PRKRA; protein ki |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | PRKRA |
| Immunogen | A synthetic peptide derived from of human PACT (phospho KR activating protein)/PRKRA (AA 302-313) |
| Positive Control | WB: C6, NIH/3T3, HeLa, K562, MOLT-4 and Jurkat cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human PACT (PKR activating protein) / PRKRA Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PACT (PKR activating protein) / PRKRA Antibody (Ab120014) - Western Blot
All lanes: Recombinant PACT (PKR activating protein) / PRKRA Antibody (Ab120014) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 33 kDa
Exposure time: 1.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202196 | Certificate of Analysis | Dec 28, 2023 | Ab120014 |
| ZJ23F1202195 | Certificate of Analysis | Dec 28, 2023 | Ab120014 |
| 1. Patel, R C RC and Sen, G C GC.. (1998) PACT, a protein activator of the interferon-induced protein kinase, PKR.. The EMBO journal, (3): [PMID:9687506] |
| 2. Ito, T T, Yang, M M and May, W S WS.. (1999) RAX, a cellular activator for double-stranded RNA-dependent protein kinase during stress signaling.. The Journal of biological chemistry, (28): [PMID:10336432] |
| 3. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 4. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 5. Lee, Yoontae Y and 5 more authors.. (2006) The role of PACT in the RNA silencing pathway.. The EMBO journal, (8): [PMID:16424907] |
| 6. Peters, Gregory A GA, Li, Shoudong S and Sen, Ganes C GC.. (2006) Phosphorylation of specific serine residues in the PKR activation domain of PACT is essential for its ability to mediate apoptosis.. The Journal of biological chemistry, (17): [PMID:16982605] |
| 7. Kok, Kin Hang KH, Ng, Ming-Him James MH, Ching, Yick-Pang YP and Jin, Dong-Yan DY.. (2007) Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA.. The Journal of biological chemistry, (15): [PMID:17452327] |
| 8. Mittelstadt, Megan M and 6 more authors.. (2008) Interaction of human tRNA-dihydrouridine synthase-2 with interferon-induced protein kinase PKR.. Nucleic acids research, [PMID:18096616] |
| 9. Camargos, Sarah S and 15 more authors.. (2008) DYT16, a novel young-onset dystonia-parkinsonism disorder: identification of a segregating mutation in the stress-response protein PRKRA.. The Lancet. Neurology, [PMID:18243799] |
| 10. Seibler, Philip P and 11 more authors.. (2008) A heterozygous frameshift mutation in PRKRA (DYT16) associated with generalised dystonia in a German patient.. The Lancet. Neurology, [PMID:18420150] |
| 11. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 12. Bennett, Richard L RL, Pan, Yu Y, Christian, Jaime J, Hui, Teng T and May, W Stratford WS.. (2012) The RAX/PACT-PKR stress response pathway promotes p53 sumoylation and activation, leading to G₁ arrest.. Cell cycle (Georgetown, Tex.), (15): [PMID:22214662] |
| 13. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 14. Luthra, Priya P and 11 more authors.. (2013) Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome.. Cell host & microbe, (17): [PMID:23870315] |
