Recombinant; Rabbit anti Human PAR2 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab120274-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab120274-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab120274-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab120274-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human PAR2 Antibody; WB; Unconjugated
| Product Name | Recombinant PAR2 Antibody - Primary antibody, specific to F2RL1, Rabbit IgG |
|---|---|
| Synonyms | Coagulation factor II receptor-like 1 | G-protein coupled receptor 11 | PAR-2 | Thrombin receptor-like 1 | Coagulation factor II receptor like 1 antibody | Coagulation factor II receptor-like 1 antibody | Coagulation factor II thrombin receptor like 1 ant |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.38 mg/mL |
| Host species | Rabbit |
| Specificity | F2RL1 |
| Immunogen | A synthetic peptide derived from human PAR2 (AA 330-397) |
| Positive Control | WB: K562, A549 and Hep G2 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human PAR2 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.38 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PAR2 Antibody (Ab120274) - Western Blot
All lanes: Recombinant PAR2 Antibody (Ab120274) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 55 kDa
Exposure time: 20.0 s
Recombinant PAR2 Antibody (Ab120274) - Western Blot
All lanes: Recombinant PAR2 Antibody (Ab120274) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 55 kDa
Exposure time: 50.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0709036 | Certificate of Analysis | Jul 31, 2024 | Ab120274 |
| ZJ24F0709035 | Certificate of Analysis | Jul 31, 2024 | Ab120274 |
| 1. Chackalamannil S, Wang Y, Greenlee WJ, Hu Z, Xia Y, Ahn HS, Boykow G, Hsieh Y, Palamanda J, Agans-Fantuzzi J et al.. (2008) Discovery of a novel, orally active himbacine-based thrombin receptor antagonist (SCH 530348) with potent antiplatelet activity.. J Med Chem, 51 (11): (3061-4). [PMID:18447380] |
| 2. Lee MC, Huang SC. (2008) Proteinase-activated receptor-1 (PAR(1)) and PAR(2) but not PAR(4) mediate contraction in human and guinea-pig gallbladders.. Neurogastroenterol Motil, 20 (4): (385-91). [PMID:18179608] |
| 3. Barry GD, Suen JY, Le GT, Cotterell A, Reid RC, Fairlie DP. (2010) Novel agonists and antagonists for human protease activated receptor 2.. J Med Chem, 53 (20): (7428-40). [PMID:20873792] |
| 4. Takizawa T, Tamiya M, Hara T, Matsumoto J, Saito N, Kanke T, Kawagoe J, Hattori Y. (2005) Abrogation of bronchial eosinophilic inflammation and attenuated eotaxin content in protease-activated receptor 2-deficient mice.. J Pharmacol Sci, 98 (1): (99-102). [PMID:15879675] |
| 5. Khoufache K, LeBouder F, Morello E, Laurent F, Riffault S, Andrade-Gordon P, Boullier S, Rousset P, Vergnolle N, Riteau B. (2009) Protective role for protease-activated receptor-2 against influenza virus pathogenesis via an IFN-gamma-dependent pathway.. J Immunol, 182 (12): (7795-802). [PMID:19494303] |
| 6. Noorbakhsh F, Tsutsui S, Vergnolle N, Boven LA, Shariat N, Vodjgani M, Warren KG, Andrade-Gordon P, Hollenberg MD, Power C. (2006) Proteinase-activated receptor 2 modulates neuroinflammation in experimental autoimmune encephalomyelitis and multiple sclerosis.. J Exp Med, 203 (2): (425-35). [PMID:16476770] |
| 7. Seeliger S, Derian CK, Vergnolle N, Bunnett NW, Nawroth R, Schmelz M, Von Der Weid PY, Buddenkotte J, Sunderkötter C, Metze D et al.. (2003) Proinflammatory role of proteinase-activated receptor-2 in humans and mice during cutaneous inflammation in vivo.. FASEB J, 17 (13): (1871-85). [PMID:14519665] |
| 8. Migliore M, Habrant D, Sasso O, Albani C, Bertozzi SM, Armirotti A, Piomelli D, Scarpelli R. (2016) Potent multitarget FAAH-COX inhibitors: Design and structure-activity relationship studies.. Eur J Med Chem, 109 (3): (216-37). [PMID:26774927] |
| 9. Cheng RKY, Fiez-Vandal C, Schlenker O, Edman K, Aggeler B, Brown DG, Brown GA, Cooke RM, Dumelin CE, Doré AS et al.. (2017) Structural insight into allosteric modulation of protease-activated receptor 2.. Nature, 545 (7652): (112-115). [PMID:28445455] |
| 10. Jiang Y, Yau MK, Lim J, Wu KC, Xu W, Suen JY, Fairlie DP. (2018) A Potent Antagonist of Protease-Activated Receptor 2 That Inhibits Multiple Signaling Functions in Human Cancer Cells.. J Pharmacol Exp Ther, 364 (2): (246-257). [PMID:29263243] |
| 11. LeSarge JC, Thibeault P, Milne M, Ramachandran R, Luyt LG. (2019) High Affinity Fluorescent Probe for Proteinase-Activated Receptor 2 (PAR2).. ACS Med Chem Lett, 10 (7): (1045-1050). [PMID:31312406] |
| 12. O'Brien PJ,Prevost N,Molino M,Hollinger MK,Woolkalis MJ,Woulfe DS,Brass LF. () Thrombin responses in human endothelial cells. Contributions from receptors other than PAR1 include the transactivation of PAR2 by thrombin-cleaved PAR1.. J Biol Chem, [PMID:10788464] |
| 13. Boitano S, Flynn AN, Schulz SM, Hoffman J, Price TJ, Vagner J.. (2011) Potent agonists of the protease activated receptor 2 (PAR2).. J Med Chem, 54 (5): (1308-1313). [PMID:21294569] |
| 14. Gandhi DM, Majewski MW, Rosas R, Kentala K, Foster TJ, Greve E, Dockendorff C.. (2018) Characterization of Protease-Activated Receptor (PAR) ligands: Parmodulins are reversible allosteric inhibitors of PAR1-driven calcium mobilization in endothelial cells.. Bioorg Med Chem, 26 (9): (2514-2529). [PMID:29685684] |
| 15. Gandhi DM, Rosas R, Greve E, Kentala K, D-R Diby N, Snyder VA, Stephans A, Yeung THW, Subramaniam S, DiMilo E, Kurtenbach KE, Arnold LA, Weiler H, Dockendorff C.. (2019) The parmodulin NRD-21 is an allosteric inhibitor of PAR1 Gq signaling with improved anti-inflammatory activity and stability.. Bioorg Med Chem, 27 (17): (3788-3796). [PMID:31320211] |
| 16. Nystedt, S S, Emilsson, K K, Larsson, A K AK, Strömbeck, B B and Sundelin, J J.. (1995) Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor 2.. European journal of biochemistry, (15): [PMID:7556175] |
| 17. Bohm, S K SK and 10 more authors.. (1996) Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2.. The Biochemical journal, (15): [PMID:8615752] |
| 18. Kahn, M M and 8 more authors.. (1996) Conserved structure and adjacent location of the thrombin receptor and protease-activated receptor 2 genes define a protease-activated receptor gene cluster.. Molecular medicine (Cambridge, Mass.), [PMID:8784787] |
| 19. Molino, M M and 9 more authors.. (1997) Interactions of mast cell tryptase with thrombin receptors and PAR-2.. The Journal of biological chemistry, (14): [PMID:9020112] |
| 20. DeFea, K A KA and 5 more authors.. (2000) beta-arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2.. The Journal of cell biology, (20): [PMID:10725339] |
| 21. Takeuchi, T T and 5 more authors.. (2000) Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates.. The Journal of biological chemistry, (25): [PMID:10831593] |
| 22. Kanke, T T and 6 more authors.. (2001) Proteinase-activated receptor-2-mediated activation of stress-activated protein kinases and inhibitory kappa B kinases in NCTC 2544 keratinocytes.. The Journal of biological chemistry, (24): [PMID:11413129] |
| 23. Sun, G G, Stacey, M A MA, Schmidt, M M, Mori, L L and Mattoli, S S.. (2001) Interaction of mite allergens Der p3 and Der p9 with protease-activated receptor-2 expressed by lung epithelial cells.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:11441110] |
| 24. Lourbakos, A A and 7 more authors.. (2001) Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion.. Infection and immunity, [PMID:11447194] |
| 25. Miike, S S, McWilliam, A S AS and Kita, H H.. (2001) Trypsin induces activation and inflammatory mediator release from human eosinophils through protease-activated receptor-2.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:11714832] |
| 26. Compton, Steven J SJ, Sandhu, Sabrina S, Wijesuriya, Suranga J SJ and Hollenberg, Morley D MD.. (2002) Glycosylation of human proteinase-activated receptor-2 (hPAR2): role in cell surface expression and signalling.. The Biochemical journal, (1): [PMID:12171601] |
| 27. Bolton, Sarah J SJ, McNulty, Clare A CA, Thomas, Rebecca J RJ, Hewitt, Colin R A CR and Wardlaw, Andrew J AJ.. (2003) Expression of and functional responses to protease-activated receptors on human eosinophils.. Journal of leukocyte biology, [PMID:12832443] |
| 28. Shpacovitch, V M VM and 13 more authors.. (2004) Agonists of proteinase-activated receptor-2 modulate human neutrophil cytokine secretion, expression of cell adhesion molecules, and migration within 3-D collagen lattices.. Journal of leukocyte biology, [PMID:15155775] |
| 29. Jacob, Claire C and 5 more authors.. (2005) c-Cbl mediates ubiquitination, degradation, and down-regulation of human protease-activated receptor 2.. The Journal of biological chemistry, (22): [PMID:15708858] |
| 30. Hansen, Kristina K KK and 8 more authors.. (2005) A major role for proteolytic activity and proteinase-activated receptor-2 in the pathogenesis of infectious colitis.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:15919826] |
| 31. Feistritzer, C C, Lenta, R R and Riewald, M M.. (2005) Protease-activated receptors-1 and -2 can mediate endothelial barrier protection: role in factor Xa signaling.. Journal of thrombosis and haemostasis : JTH, [PMID:16359518] |
| 32. Luo, Weibo W, Wang, Yingfei Y, Hanck, Theodor T, Stricker, Rolf R and Reiser, Georg G.. (2006) Jab1, a novel protease-activated receptor-2 (PAR-2)-interacting protein, is involved in PAR-2-induced activation of activator protein-1.. The Journal of biological chemistry, (24): [PMID:16410250] |
| 33. Csernok, Elena E and 8 more authors.. (2006) Wegener autoantigen induces maturation of dendritic cells and licenses them for Th1 priming via the protease-activated receptor-2 pathway.. Blood, (1): [PMID:16478888] |
| 34. Winter, Michael C MC, Shasby, Sandra S SS, Ries, Dana R DR and Shasby, D Michael DM.. (2006) PAR2 activation interrupts E-cadherin adhesion and compromises the airway epithelial barrier: protective effect of beta-agonists.. American journal of physiology. Lung cellular and molecular physiology, [PMID:16714334] |
| 35. Chiu, Li-Li LL, Perng, Diahn-Warng DW, Yu, Chia-Hsien CH, Su, Song-Nan SN and Chow, Lu-Ping LP.. (2007) Mold allergen, pen C 13, induces IL-8 expression in human airway epithelial cells by activating protease-activated receptor 1 and 2.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:17404307] |
| 36. Zoudilova, Maria M and 5 more authors.. (2007) Beta-arrestin-dependent regulation of the cofilin pathway downstream of protease-activated receptor-2.. The Journal of biological chemistry, (13): [PMID:17500066] |
| 37. Luo, Weibo W, Wang, Yingfei Y and Reiser, Georg G.. (2007) p24A, a type I transmembrane protein, controls ARF1-dependent resensitization of protease-activated receptor-2 by influence on receptor trafficking.. The Journal of biological chemistry, (12): [PMID:17693410] |
| 38. Goon Goh, Fui F and 5 more authors.. (2008) G-protein-dependent and -independent pathways regulate proteinase-activated receptor-2 mediated p65 NFkappaB serine 536 phosphorylation in human keratinocytes.. Cellular signalling, [PMID:18424071] |
| 39. Feld, Micha M and 7 more authors.. (2008) Agonists of proteinase-activated receptor-2 enhance IFN-gamma-inducible effects on human monocytes: role in influenza A infection.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:18453611] |
| 40. Hong, Jeong Hee JH and 7 more authors.. (2008) Chitinase activates protease-activated receptor-2 in human airway epithelial cells.. American journal of respiratory cell and molecular biology, [PMID:18474671] |
| 41. Rallabhandi, Prasad P and 7 more authors.. (2008) Analysis of proteinase-activated receptor 2 and TLR4 signal transduction: a novel paradigm for receptor cooperativity.. The Journal of biological chemistry, (5): [PMID:18622013] |
| 42. Hasdemir, Burcu B, Murphy, Jane E JE, Cottrell, Graeme S GS and Bunnett, Nigel W NW.. (2009) Endosomal deubiquitinating enzymes control ubiquitination and down-regulation of protease-activated receptor 2.. The Journal of biological chemistry, (9): [PMID:19684015] |
| 43. McIntosh, Kathryn K and 6 more authors.. (2010) Proteinase-activated receptor-2 mediated inhibition of TNFalpha-stimulated JNK activation - A novel paradigm for G(q/11) linked GPCRs.. Cellular signalling, [PMID:19781631] |
| 44. Ricks, Tiffany K TK and Trejo, JoAnn J.. (2009) Phosphorylation of protease-activated receptor-2 differentially regulates desensitization and internalization.. The Journal of biological chemistry, (4): [PMID:19815543] |
| 45. Matsuwaki, Yoshinori Y and 10 more authors.. (2009) Recognition of fungal protease activities induces cellular activation and eosinophil-derived neurotoxin release in human eosinophils.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:19864598] |
| 46. Nhu, Q M QM and 7 more authors.. (2010) Novel signaling interactions between proteinase-activated receptor 2 and Toll-like receptors in vitro and in vivo.. Mucosal immunology, [PMID:19865078] |
| 47. Holzhausen, M M and 5 more authors.. (2010) Protease-activated receptor-2 (PAR(2)) in human periodontitis.. Journal of dental research, [PMID:20530726] |
| 48. Bae, Jong-Sup JS, Yang, Likui L and Rezaie, Alireza R AR.. (2010) Factor X/Xa elicits protective signaling responses in endothelial cells directly via PAR-2 and indirectly via endothelial protein C receptor-dependent recruitment of PAR-1.. The Journal of biological chemistry, (5): [PMID:20826780] |
| 49. Shpacovitch, Victoria M VM and 10 more authors.. (2011) Role of proteinase-activated receptor-2 in anti-bacterial and immunomodulatory effects of interferon-γ on human neutrophils and monocytes.. Immunology, [PMID:21501162] |
| 50. Botham, Andrew A and 5 more authors.. (2011) Palmitoylation of human proteinase-activated receptor-2 differentially regulates receptor-triggered ERK1/2 activation, calcium signalling and endocytosis.. The Biochemical journal, (1): [PMID:21627585] |
| 51. Kuckleburg, Christopher J CJ and Newman, Peter J PJ.. (2013) Neutrophil proteinase 3 acts on protease-activated receptor-2 to enhance vascular endothelial cell barrier function.. Arteriosclerosis, thrombosis, and vascular biology, [PMID:23202369] |
