A synthetic peptide derived from human PARP 2 (AA 501-583)
Positive Control
WB: Raji, Jurkat, Hep G2 and A549 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human PARP2 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/1000-1/2000 Protein Function: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant PARP2 Antibody (Ab120397) - Western Blot All lanes: Recombinant PARP2 Antibody (Ab120397) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 66 kDa Observed band size: 59 kDa Exposure time: 37.8 s
Recombinant PARP2 Antibody (Ab120397) - Western Blot All lanes: Recombinant PARP2 Antibody (Ab120397) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 66 kDa Observed band size: 59 kDa Exposure time: 37.8 s