Recombinant (RR704); Rabbit anti Human PCNA Antibody; WB, Flow, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab120681-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $49.90 | |
| Ab120681-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $147.90 | |
| Ab120681-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab120681-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,407.90 |
Recombinant (RR704); Rabbit anti Human PCNA Antibody; WB, Flow, IP; Unconjugated
| Product Name | Recombinant PCNA Antibody - Primary antibody, specific to PCNA, Rabbit IgG |
|---|---|
| Synonyms | ATLD2 antibody; cb16 antibody; Cyclin antibody; DNA polymerase delta auxiliary protein antibody; etID36690.10 antibody; fa28e03 antibody; fb36g03 antibody; HGCN8729 antibody; MGC8367 antibody; Mutagen-sensitive 209 protein antibody; OTTHUMP00000030189 ant |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | PCNA |
| Immunogen | A synthetic peptide corresponding to residues of human PCNA was used as an immunogen (AA 100-200). |
| Positive Control | WB: A431, HEK293, MCF7, HeLa, Hep G2, NIH/3T3, C6, K562, Jurkat and HUVEC cell lysates. Flow: HeLa cells. IP: HeLa whole cell lysate. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human PCNA Antibody, Recombinant (RR704), could be used for WB, Flow, IF/ICC, IP and so on. IF/ICC: 1/1000 |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PCNA Antibody (Ab120681) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 14.1 s
Recombinant PCNA Antibody (Ab120681) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant PCNA Antibody (Ab120681) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant PCNA Antibody (Ab120681) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant PCNA Antibody (Ab120681) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant PCNA Antibody (Ab120681) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant PCNA Antibody (Ab120681) - IP
PCNA was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant PCNA Antibody (Ab120681) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant PCNA Antibody (Ab120681) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant PCNA Antibody (Ab120681) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
1/1000 - 1/2000
|
| IF/ICC | 1/1000 |
| IP |
1/50
|
| Flow | 1/800 - 1/2000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901113 | Certificate of Analysis | Sep 24, 2023 | Ab120681 |
| ZJ23F0901112 | Certificate of Analysis | Sep 24, 2023 | Ab120681 |
