| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab121167-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab121167-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $209.90 | |
| Ab121167-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab121167-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human Pellino 1 Antibody; WB; Unconjugated
| Product Name | Recombinant Pellino 1 Antibody - Primary antibody, specific to PELI1, Rabbit IgG | |
|---|---|---|
| Synonyms | EC 2.3.2.27 | Pellino-1 | Pellino-related intracellular-signaling molecule | RING-type E3 ubiquitin transferase pellino homolog 1 | E3 ubiquitin-protein ligase pellino homolog 1 antibody | PELI1 antibody | PELI1_HUMAN antibody | Pellino E3 ubiquitin prote | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL | |
| Host species | Rabbit | |
| Specificity | PELI1 | |
| Immunogen | Recombinant protein of human Pellino 1 (AA 1-418) | |
| Positive Control | WB: Ramos and NIH/3T3 cell lysates. | |
| Conjugation | Unconjugated | |
| Grade | ExactAb™, Recombinant, Validated | |
| Product Description | Rabbit anti Human Pellino 1 Antibody, Recombinant, could be used for WB and so on. Application:
Protein Function Scaffold protein involved in the IL-1 signaling pathway via its interaction with the complex containing IRAK kinases and TRAF6. Required for NF-kappa-B activation and IL-8 gene expression in response to IL-1. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Pellino 1 Antibody (Ab121167) - Western Blot
All lanes: Recombinant Pellino 1 Antibody (Ab121167) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 100.3 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0606462 | Certificate of Analysis | Jun 04, 2024 | Ab121167 |
| ZJ24F0606461 | Certificate of Analysis | Jun 04, 2024 | Ab121167 |
| 1. Rich, T T, Allen, R L RL, Lucas, A M AM, Stewart, A A and Trowsdale, J J.. (2000) Pellino-related sequences from Caenorhabditis elegans and Homo sapiens.. Immunogenetics, [PMID:11132151] |
| 2. Resch, K K, Jockusch, H H and Schmitt-John, T T.. (2001) Assignment of homologous genes, Peli1/PELI1 and Peli2/PELI2, for the Pelle adaptor protein Pellino to mouse chromosomes 11 and 14 and human chromosomes 2p13.3 and 14q21, respectively, by physical and radiation hybrid mapping.. Cytogenetics and cell genetics, [PMID:11306823] |
| 3. Jiang, Zhengfan Z and 5 more authors.. (2003) Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex.. The Journal of biological chemistry, (28): [PMID:12496252] |
| 4. Jensen, Liselotte E LE and Whitehead, Alexander S AS.. (2003) Pellino2 activates the mitogen activated protein kinase pathway.. FEBS letters, (19): [PMID:12804775] |
| 5. Choi, Kyung-Chul KC and 9 more authors.. (2006) Smad6 negatively regulates interleukin 1-receptor-Toll-like receptor signaling through direct interaction with the adaptor Pellino-1.. Nature immunology, [PMID:16951688] |
| 6. Butler, Marion P MP, Hanly, Jennifer A JA and Moynagh, Paul N PN.. (2007) Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for PELLINO proteins being ubiquitin-protein isopeptide ligases.. The Journal of biological chemistry, (12): [PMID:17675297] |
| 7. Ordureau, Alban A and 6 more authors.. (2008) The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1.. The Biochemical journal, (1): [PMID:17997719] |
| 8. Choi, Seung-Won SW and 10 more authors.. (2018) PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation.. Molecular cell, (7): [PMID:29883609] |
