Recombinant Peroxiredoxin 3/PRDX3 Antibody - Primary antibody, specific to Peroxiredoxin 3/PRDX3, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab121327
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab121327-10μl
10μl
In stock
$76.90
Ab121327-50μl
50μl
In stock
$229.90
Ab121327-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$369.90
Ab121327-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human Peroxiredoxin 3/PRDX3 Antibody; WB, ICC, IF, IHC; Unconjugated

Basic Description

Product NameRecombinant Peroxiredoxin 3/PRDX3 Antibody - Primary antibody, specific to Peroxiredoxin 3/PRDX3, Rabbit IgG
SynonymsAOP1 | PPPCD | PRO1748 | SCAR32 | SP-22 | Antioxidant protein 1MGC104387 | AOP-1 | AOP-1MGC24293 | AOP1PRO1748 | EC 1.11.1.15 | HBC189 | MER5 | Peroxiredoxin 3 | Peroxiredoxin III | peroxiredoxin-3 | PRDX3 | Protein MER5 homolog | prx-III | thioredoxin-de
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityPeroxiredoxin 3/PRDX3
ImmunogenA synthetic peptide derived from human Peroxiredoxin 3/PRDX3 (AA 243-256)
Positive ControlWB: HeLa, A431, MOLT-4, Hep G2 and MCF7 cell lysates. ICC/IF: HeLa cells. IHC: Human cholangiocarcinoma tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.Rabbit anti Human Peroxiredoxin 3/PRDX3 Antibody, Recombinant, could be used for WB, ICC, IF, IHC and so on.Application
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
IHC: 1/50-1/100
Protein Function
Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - Western Blot
All lanes: Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 23 kDa
Exposure time: 8.6 s

Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - IF/ICC
Immunocytochemistry analysis of Peroxiredoxin 3 (green) in HeLa using Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327), and cells were counterstained with DAPI (blue).

Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

PRDX3 Tbio Thioredoxin-dependent peroxide reductase, mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
PRDX3 Tbio Thioredoxin-dependent peroxide reductase, mitochondrial (6 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303040Certificate of AnalysisMar 08, 2024 Ab121327
ZJ23F1202161Certificate of AnalysisDec 20, 2023 Ab121327
ZJ23F1202162Certificate of AnalysisDec 20, 2023 Ab121327

Related Documents

References

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3. Hochstrasser, D F DF and 9 more authors..  (1992)  Human liver protein map: a reference database established by microsequencing and gel comparison..  Electrophoresis,      [PMID:1286669]
4. Tsuji, K K, Copeland, N G NG, Jenkins, N A NA and Obinata, M M..  (1995)  Mammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coli..  The Biochemical journal,    (15):   [PMID:7733872]
5. Wagner, Elsa E and 6 more authors..  (2002)  A method for detection of overoxidation of cysteines: peroxiredoxins are oxidized in vivo at the active-site cysteine during oxidative stress..  The Biochemical journal,    (15):   [PMID:12059788]
6. Masaki, Megumi M, Ikeda, Atsushi A, Shiraki, Eriko E, Oka, Shogo S and Kawasaki, Toshisuke T..  (2003)  Mixed lineage kinase LZK and antioxidant protein-1 activate NF-kappaB synergistically..  European journal of biochemistry,      [PMID:12492477]
7. Deloukas, P P and 135 more authors..  (2004)  The DNA sequence and comparative analysis of human chromosome 10..  Nature,    (27):   [PMID:15164054]
8. Liu, Lingling L and 8 more authors..  (2005)  RPK118, a PX domain-containing protein, interacts with peroxiredoxin-3 through pseudo-kinase domains..  Molecules and cells,    (28):   [PMID:15750338]
9. Cao, Zhenbo Z, Bhella, David D and Lindsay, J Gordon JG..  (2007)  Reconstitution of the mitochondrial PrxIII antioxidant defence pathway: general properties and factors affecting PrxIII activity and oligomeric state..  Journal of molecular biology,    (28):   [PMID:17707404]
10. Cox, Andrew G AG, Peskin, Alexander V AV, Paton, Louise N LN, Winterbourn, Christine C CC and Hampton, Mark B MB..  (2009)  Redox potential and peroxide reactivity of human peroxiredoxin 3..  Biochemistry,    (14):   [PMID:19462976]
11. Vaz Meirelles, Gabriela G and 5 more authors..  (2010)  Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome..  Journal of proteome research,    (3):   [PMID:20873783]
12. Angeles, Dario C DC and 10 more authors..  (2011)  Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death..  Human mutation,      [PMID:21850687]
13. Yewdall, N Amy NA and 5 more authors..  (2018)  Quaternary structure influences the peroxidase activity of peroxiredoxin 3..  Biochemical and biophysical research communications,    (4):   [PMID:29438714]
14. Alió Del Barrio, Jorge L and 10 more authors..  (2020)  Punctiform and Polychromatic Pre-Descemet Corneal Dystrophy: Clinical Evaluation and Identification of the Genetic Basis..  American journal of ophthalmology,      [PMID:31782998]

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