Recombinant; Rabbit anti Human Peroxiredoxin 4 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab121332-10μl | 10μl | In stock | $56.90 | |
| Ab121332-50μl | 50μl | In stock | $169.90 | |
| Ab121332-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $269.90 | |
| Ab121332-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human Peroxiredoxin 4 Antibody; WB; Unconjugated
| Product Name | Recombinant Peroxiredoxin 4 Antibody - Primary antibody, specific to Peroxiredoxin 4, Rabbit IgG |
|---|---|
| Synonyms | AOE37-2 | AOE372 | HEL-S-97n | PRX-4 | Antioxidant enzyme AOE372 | AOE37-2PRX-4 | EC 1.11.1.15 | Peroxiredoxin 4 | Peroxiredoxin IV | peroxiredoxin-4 | PRDX4 | Prx4 | prx-IV | thioredoxin peroxidase (antioxidant enzyme) | Thioredoxin peroxidase AO372 | Th |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | Peroxiredoxin 4 |
| Immunogen | A synthetic peptide derived from human Peroxiredoxin 4 (AA 70-96). |
| Positive Control | WB: HeLa, HEK293, Hep G2, K562, Jurkat, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Peroxiredoxin 4 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | Kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Peroxiredoxin 4 Antibody (Ab121332) - Western Blot
All lanes: Recombinant Peroxiredoxin 4 Antibody (Ab121332) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 27 kDa
Exposure time: 52.9 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303538 | Certificate of Analysis | Mar 21, 2024 | Ab121332 |
| ZJ24F0303539 | Certificate of Analysis | Mar 21, 2024 | Ab121332 |
| 1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 2. Jin, D Y DY, Chae, H Z HZ, Rhee, S G SG and Jeang, K T KT.. (1997) Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation.. The Journal of biological chemistry, (5): [PMID:9388242] |
| 3. Wagner, Elsa E and 6 more authors.. (2002) A method for detection of overoxidation of cysteines: peroxiredoxins are oxidized in vivo at the active-site cysteine during oxidative stress.. The Biochemical journal, (15): [PMID:12059788] |
| 4. Tavender, Timothy J TJ, Sheppard, Alyson M AM and Bulleid, Neil J NJ.. (2008) Peroxiredoxin IV is an endoplasmic reticulum-localized enzyme forming oligomeric complexes in human cells.. The Biochemical journal, (1): [PMID:18052930] |
| 5. Wang, Xi X, Wang, Likun L, Wang, Xi'e X, Sun, Fei F and Wang, Chih-chen CC.. (2012) Structural insights into the peroxidase activity and inactivation of human peroxiredoxin 4.. The Biochemical journal, (1): [PMID:21916849] |
| 6. Cao, Zhenbo Z, Tavender, Timothy J TJ, Roszak, Aleksander W AW, Cogdell, Richard J RJ and Bulleid, Neil J NJ.. (2011) Crystal structure of reduced and of oxidized peroxiredoxin IV enzyme reveals a stable oxidized decamer and a non-disulfide-bonded intermediate in the catalytic cycle.. The Journal of biological chemistry, (9): [PMID:21994946] |
