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Size

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Ab121596-10μl

10μl

In stock

$66.90

Ab121596-50μl

50μl

In stock

$199.90

Ab121596-100μl

100μl

Available within 8-12 weeks(?)

$299.90

Ab121596-1ml

1ml

Available within 8-12 weeks(?)

$2,699.90

Basic Description

Product Name	Recombinant PHF8 Antibody - Primary antibody, specific to PHF8, Rabbit IgG
Synonyms	PHD finger protein 8; PHD finger protein 8; Histone lysine demethylase PHF8; PHD finger protein 8; PHF8_HUMAN; ZNF422; Histone lysine demethylase PHF8 antibody; JHDM1F antibody; Jumonji C domain containing histone demethylase 1F antibody; MRXSSD antibody;
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species	Rabbit
Specificity	PHF8
Immunogen	A synthetic peptide derived from human PHF8 (AA 800-860)
Positive Control	WB: Jurkat, MOLT-4, HeLa, HEK293, LNCaP, RAW 264.7 and C6 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human PHF8 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Histone lysine demethylase with selectivity for the di-and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant PHF8 Antibody (Ab121596) - Western Blot
All lanes: Recombinant PHF8 Antibody (Ab121596) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 102, 139 kDa
Exposure time: 18.4 s

Associated Targets

PHF8 Tchem Histone lysine demethylase PHF8 0 Activities

Discover Target: PHF8

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

AOPEP Tbio Aminopeptidase O 0 Activities

Discover Target: AOPEP

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505723	Certificate of Analysis	May 16, 2024	Ab121596
ZJ24F0505722	Certificate of Analysis	May 16, 2024	Ab121596

References

1. Rose NR, Woon EC, Tumber A, Walport LJ, Chowdhury R, Li XS, King ON, Lejeune C, Ng SS, Krojer T et al.. (2012) Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone demethylases.. J Med Chem, 55 (14): (6639-43). [PMID:22724510]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Bhushan B, Erdmann A, Zhang Y, Belle R, Johannson C, Oppermann U, Hopkinson RJ, Schofield CJ, Kawamura A.. (2018) Investigations on small molecule inhibitors targeting the histone H3K4 tri-methyllysine binding PHD-finger of JmjC histone demethylases.. Bioorg Med Chem, 26 (11): (2984-2991). [PMID:29764755]
4. Kikuno, R R and 8 more authors.. (1999) Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.. DNA research : an international journal for rapid publication of reports on genes and genomes, (30): [PMID:10470851]
5. Nakajima, Daisuke D and 5 more authors.. (2002) Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.. DNA research : an international journal for rapid publication of reports on genes and genomes, (30): [PMID:12168954]
6. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651]
7. Laumonnier, F F and 17 more authors.. (2005) Mutations in PHF8 are associated with X linked mental retardation and cleft lip/cleft palate.. Journal of medical genetics, [PMID:16199551]
8. Koivisto, A M AM and 5 more authors.. (2007) Screening of mutations in the PHF8 gene and identification of a novel mutation in a Finnish family with XLMR and cleft lip/cleft palate.. Clinical genetics, [PMID:17661819]
9. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
10. Loenarz, Christoph C and 7 more authors.. (2010) PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nepsilon-dimethyl lysine demethylase.. Human molecular genetics, (15): [PMID:19843542]
11. Horton, John R JR and 5 more authors.. (2010) Enzymatic and structural insights for substrate specificity of a family of jumonji histone lysine demethylases.. Nature structural & molecular biology, [PMID:20023638]
12. Yue, Wyatt W WW and 8 more authors.. (2010) Crystal structure of the PHF8 Jumonji domain, an Nepsilon-methyl lysine demethylase.. FEBS letters, (19): [PMID:20067792]
13. Yu, Lin L and 10 more authors.. (2010) Structural insights into a novel histone demethylase PHF8.. Cell research, [PMID:20101266]
14. Feng, Weijun W, Yonezawa, Masato M, Ye, Jing J, Jenuwein, Thomas T and Grummt, Ingrid I.. (2010) PHF8 activates transcription of rRNA genes through H3K4me3 binding and H3K9me1/2 demethylation.. Nature structural & molecular biology, [PMID:20208542]
15. Kleine-Kohlbrecher, Daniela D and 10 more authors.. (2010) A functional link between the histone demethylase PHF8 and the transcription factor ZNF711 in X-linked mental retardation.. Molecular cell, (23): [PMID:20346720]
16. Fortschegger, Klaus K and 5 more authors.. (2010) PHF8 targets histone methylation and RNA polymerase II to activate transcription.. Molecular and cellular biology, [PMID:20421419]
17. Zhu, Ziqi Z and 14 more authors.. (2010) PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis.. Cell research, [PMID:20531378]
18. Qiu, Jihui J and 7 more authors.. (2010) The X-linked mental retardation gene PHF8 is a histone demethylase involved in neuronal differentiation.. Cell research, [PMID:20548336]
19. Qi, Hank H HH and 16 more authors.. (2010) Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development.. Nature, (22): [PMID:20622853]
20. Liu, Wen W and 13 more authors.. (2010) PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.. Nature, (22): [PMID:20622854]
21. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
22. Nava, C C and 26 more authors.. (2012) Analysis of the chromosome X exome in patients with autism spectrum disorders identified novel candidate genes, including TMLHE.. Translational psychiatry, (23): [PMID:23092983]
23. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
24. Tumber, Anthony A and 27 more authors.. (2017) Potent and Selective KDM5 Inhibitor Stops Cellular Demethylation of H3K4me3 at Transcription Start Sites and Proliferation of MM1S Myeloma Cells.. Cell chemical biology, (16): [PMID:28262558]
25. Poeta, Loredana and 17 more authors.. (2019) Histone demethylase KDM5C is a SAHA-sensitive central hub at the crossroads of transcriptional axes involved in multiple neurodevelopmental disorders.. Human molecular genetics, (15): [PMID:31691806]

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Recombinant PHF8 Antibody - Primary antibody, specific to PHF8, Rabbit IgG