| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab121596-10μl | 10μl | In stock | $66.90 | |
| Ab121596-50μl | 50μl | In stock | $199.90 | |
| Ab121596-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab121596-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human PHF8 Antibody; WB; Unconjugated
| Product Name | Recombinant PHF8 Antibody - Primary antibody, specific to PHF8, Rabbit IgG |
|---|---|
| Synonyms | PHD finger protein 8 | Histone lysine demethylase PHF8 | PHF8_HUMAN | ZNF422 | Histone lysine demethylase PHF8 antibody | JHDM1F antibody | Jumonji C domain containing histone demethylase 1F antibody | MRXSSD antibody | PHD finger protein 8 antibody | PHF |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | PHF8 |
| Immunogen | A synthetic peptide derived from human PHF8 (AA 800-860) |
| Positive Control | WB: Jurkat, MOLT-4, HeLa, HEK293, LNCaP, RAW 264.7 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human PHF8 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PHF8 Antibody (Ab121596) - Western Blot
All lanes: Recombinant PHF8 Antibody (Ab121596) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 102, 139 kDa
Exposure time: 18.4 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505723 | Certificate of Analysis | May 16, 2024 | Ab121596 |
| ZJ24F0505722 | Certificate of Analysis | May 16, 2024 | Ab121596 |
| 1. Rose NR, Woon EC, Tumber A, Walport LJ, Chowdhury R, Li XS, King ON, Lejeune C, Ng SS, Krojer T et al.. (2012) Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone demethylases.. J Med Chem, 55 (14): (6639-43). [PMID:22724510] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Bhushan B, Erdmann A, Zhang Y, Belle R, Johannson C, Oppermann U, Hopkinson RJ, Schofield CJ, Kawamura A.. (2018) Investigations on small molecule inhibitors targeting the histone H3K4 tri-methyllysine binding PHD-finger of JmjC histone demethylases.. Bioorg Med Chem, 26 (11): (2984-2991). [PMID:29764755] |
| 4. Kikuno, R R and 8 more authors.. (1999) Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.. DNA research : an international journal for rapid publication of reports on genes and genomes, (30): [PMID:10470851] |
| 5. Nakajima, Daisuke D and 5 more authors.. (2002) Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.. DNA research : an international journal for rapid publication of reports on genes and genomes, (30): [PMID:12168954] |
| 6. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651] |
| 7. Laumonnier, F F and 17 more authors.. (2005) Mutations in PHF8 are associated with X linked mental retardation and cleft lip/cleft palate.. Journal of medical genetics, [PMID:16199551] |
| 8. Koivisto, A M AM and 5 more authors.. (2007) Screening of mutations in the PHF8 gene and identification of a novel mutation in a Finnish family with XLMR and cleft lip/cleft palate.. Clinical genetics, [PMID:17661819] |
| 9. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 10. Loenarz, Christoph C and 7 more authors.. (2010) PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nepsilon-dimethyl lysine demethylase.. Human molecular genetics, (15): [PMID:19843542] |
| 11. Horton, John R JR and 5 more authors.. (2010) Enzymatic and structural insights for substrate specificity of a family of jumonji histone lysine demethylases.. Nature structural & molecular biology, [PMID:20023638] |
| 12. Yue, Wyatt W WW and 8 more authors.. (2010) Crystal structure of the PHF8 Jumonji domain, an Nepsilon-methyl lysine demethylase.. FEBS letters, (19): [PMID:20067792] |
| 13. Yu, Lin L and 10 more authors.. (2010) Structural insights into a novel histone demethylase PHF8.. Cell research, [PMID:20101266] |
| 14. Feng, Weijun W, Yonezawa, Masato M, Ye, Jing J, Jenuwein, Thomas T and Grummt, Ingrid I.. (2010) PHF8 activates transcription of rRNA genes through H3K4me3 binding and H3K9me1/2 demethylation.. Nature structural & molecular biology, [PMID:20208542] |
| 15. Kleine-Kohlbrecher, Daniela D and 10 more authors.. (2010) A functional link between the histone demethylase PHF8 and the transcription factor ZNF711 in X-linked mental retardation.. Molecular cell, (23): [PMID:20346720] |
| 16. Fortschegger, Klaus K and 5 more authors.. (2010) PHF8 targets histone methylation and RNA polymerase II to activate transcription.. Molecular and cellular biology, [PMID:20421419] |
| 17. Zhu, Ziqi Z and 14 more authors.. (2010) PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis.. Cell research, [PMID:20531378] |
| 18. Qiu, Jihui J and 7 more authors.. (2010) The X-linked mental retardation gene PHF8 is a histone demethylase involved in neuronal differentiation.. Cell research, [PMID:20548336] |
| 19. Qi, Hank H HH and 16 more authors.. (2010) Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development.. Nature, (22): [PMID:20622853] |
| 20. Liu, Wen W and 13 more authors.. (2010) PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.. Nature, (22): [PMID:20622854] |
| 21. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 22. Nava, C C and 26 more authors.. (2012) Analysis of the chromosome X exome in patients with autism spectrum disorders identified novel candidate genes, including TMLHE.. Translational psychiatry, (23): [PMID:23092983] |
| 23. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 24. Tumber, Anthony A and 27 more authors.. (2017) Potent and Selective KDM5 Inhibitor Stops Cellular Demethylation of H3K4me3 at Transcription Start Sites and Proliferation of MM1S Myeloma Cells.. Cell chemical biology, (16): [PMID:28262558] |
| 25. Poeta, Loredana and 17 more authors.. (2019) Histone demethylase KDM5C is a SAHA-sensitive central hub at the crossroads of transcriptional axes involved in multiple neurodevelopmental disorders.. Human molecular genetics, (15): [PMID:31691806] |
