Recombinant PIAS1 Antibody - Primary antibody, specific to PIAS1, Rabbit IgG

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab121809
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab121809-10μl
10μl
In stock
$76.90
Ab121809-50μl
50μl
In stock
$219.90
Ab121809-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab121809-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human PIAS1 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant PIAS1 Antibody - Primary antibody, specific to PIAS1, Rabbit IgG
SynonymsAR interacting protein antibody | DDXBP1 antibody | DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody | DEAD/H box binding protein 1 antibody | DEAD/H box-binding protein 1 antibody | E3 SUMO-protein ligase PIAS1 antibody | GBP antibody | Gu bin
Specifications & PurityExactAb™, Validated, Recombinant, 0.9 mg/mL
Host speciesRabbit
SpecificityPIAS1
ImmunogenA synthetic peptide derived from human PIAS1 (AA 63-117).
Positive ControlWB: MOLT-4, K562, HeLa, MCF7 and RAW 264.7 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human PIAS1 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500 - 1/2000
Protein Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
Post-translational
Sumoylated.
Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.9 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant PIAS1 Antibody (Ab121809) - Western Blot
All lanes: Recombinant PIAS1 Antibody (Ab121809) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 72 kDa
Observed band size: 75 kDa
Exposure time: 132.0 s

Recombinant PIAS1 Antibody (Ab121809) - Western Blot
All lanes: Recombinant PIAS1 Antibody (Ab121809) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 72 kDa
Observed band size: 75 kDa
Exposure time: 168.9 s

Associated Targets(Human)

PIAS1 Tbio E3 SUMO-protein ligase PIAS1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303544Certificate of AnalysisMar 21, 2024 Ab121809
ZJ24F0303545Certificate of AnalysisMar 21, 2024 Ab121809

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