Recombinant PIAS1 Antibody - Primary antibody, specific to PIAS1, Rabbit IgG
Synonyms
AR interacting protein antibody; DDXBP1 antibody; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody; DEAD/H box binding protein 1 antibody; DEAD/H box-binding protein 1 antibody; E3 SUMO-protein ligase PIAS1 antibody; GBP antibody; Gu binding pr
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.9 mg/mL
Host species
Rabbit
Specificity
PIAS1
Immunogen
A synthetic peptide derived from human PIAS1 (AA 63-117).
Positive Control
WB: MOLT-4, K562, HeLa, MCF7 and RAW 264.7 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human PIAS1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 - 1/2000 Protein Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Post-translational Sumoylated. Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.9 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant PIAS1 Antibody (Ab121809) - Western Blot All lanes: Recombinant PIAS1 Antibody (Ab121809) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 72 kDa Observed band size: 75 kDa Exposure time: 132.0 s
Recombinant PIAS1 Antibody (Ab121809) - Western Blot All lanes: Recombinant PIAS1 Antibody (Ab121809) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 72 kDa Observed band size: 75 kDa Exposure time: 168.9 s