Recombinant; Rabbit anti Human PIAS2 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab121813-10μl | 10μl | In stock | $76.90 | |
| Ab121813-50μl | 50μl | In stock | $219.90 | |
| Ab121813-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab121813-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human PIAS2 Antibody; WB; Unconjugated
| Product Name | Recombinant PIAS2 Antibody - Primary antibody, specific to PIAS2, Rabbit IgG |
|---|---|
| Synonyms | Androgen receptor interacting protein 3 antibody; Androgen receptor-interacting protein 3 antibody; ARIP3 antibody; DAB2 interacting protein antibody; DAB2-interacting protein antibody; DIP antibody; E3 SUMO protein ligase PIAS2 antibody; E3 SUMO-protein |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 3.0 mg/mL |
| Host species | Rabbit |
| Specificity | PIAS2 |
| Immunogen | A synthetic peptide derived from human PIAS2 (AA 114-150). |
| Positive Control | WB: Hep G2 and HEK293 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human PIAS2 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 3.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PIAS2 Antibody (Ab121813) - Western Blot
All lanes: Recombinant PIAS2 Antibody (Ab121813) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 61 kDa
Exposure time: 13.5 s
Recombinant PIAS2 Antibody (Ab121813) - Western Blot
All lanes: Recombinant PIAS2 Antibody (Ab121813) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 71 kDa
Exposure time: 100.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303546 | Certificate of Analysis | Mar 21, 2024 | Ab121813 |
| ZJ24F0303547 | Certificate of Analysis | Mar 21, 2024 | Ab121813 |
| 1. Liu, B B and 6 more authors.. (1998) Inhibition of Stat1-mediated gene activation by PIAS1.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:9724754] |
| 2. Moilanen, A M AM and 6 more authors.. (1999) A testis-specific androgen receptor coregulator that belongs to a novel family of nuclear proteins.. The Journal of biological chemistry, (5): [PMID:9920921] |
| 3. Minty, A A, Dumont, X X, Kaghad, M M and Caput, D D.. (2000) Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1 interaction motif.. The Journal of biological chemistry, (17): [PMID:10961991] |
| 4. Gross, M M and 5 more authors.. (2001) Distinct effects of PIAS proteins on androgen-mediated gene activation in prostate cancer cells.. Oncogene, (28): [PMID:11439351] |
| 5. Takahashi, K K and 5 more authors.. (2001) DJ-1 positively regulates the androgen receptor by impairing the binding of PIASx alpha to the receptor.. The Journal of biological chemistry, (5): [PMID:11477070] |
| 6. Schmidt, Darja D and Müller, Stefan S.. (2002) Members of the PIAS family act as SUMO ligases for c-Jun and p53 and repress p53 activity.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:11867732] |
| 7. Rui, Hong-Liang HL and 5 more authors.. (2002) SUMO-1 modification of the C-terminal KVEKVD of Axin is required for JNK activation but has no effect on Wnt signaling.. The Journal of biological chemistry, (8): [PMID:12223491] |
| 8. Miyauchi, Yasuhiro Y, Yogosawa, Satomi S, Honda, Reiko R, Nishida, Tamotsu T and Yasuda, Hideyo H.. (2002) Sumoylation of Mdm2 by protein inhibitor of activated STAT (PIAS) and RanBP2 enzymes.. The Journal of biological chemistry, (20): [PMID:12393906] |
| 9. Arora, Taruna T and 7 more authors.. (2003) PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4.. The Journal of biological chemistry, (13): [PMID:12716907] |
| 10. Van Dyck, Frederik F, Delvaux, Els L D EL, Van de Ven, Wim J M WJ and Chavez, Marcela V MV.. (2004) Repression of the Transactivating Capacity of the Oncoprotein PLAG1 by SUMOylation.. The Journal of biological chemistry, (20): [PMID:15208321] |
| 11. Zheng, Ying Y, Zhou, Zuo-Min ZM, Yin, Lan-Lan LL, Li, Jian-Min JM and Sha, Jia-Hao JH.. (2004) Molecular cloning and characterization of a novel splicing variant of PIASx.. Acta pharmacologica Sinica, [PMID:15301740] |
| 12. Yang, Shen-Hsi SH and Sharrocks, Andrew D AD.. (2005) PIASx acts as an Elk-1 coactivator by facilitating derepression.. The EMBO journal, (15): [PMID:15920481] |
| 13. Shinbo, Y Y and 8 more authors.. (2006) Proper SUMO-1 conjugation is essential to DJ-1 to exert its full activities.. Cell death and differentiation, [PMID:15976810] |
| 14. Nusbaum, Chad C and 54 more authors.. (2005) DNA sequence and analysis of human chromosome 18.. Nature, (22): [PMID:16177791] |
| 15. Song, Jing J, Zhang, Ziming Z, Hu, Weidong W and Chen, Yuan Y.. (2005) Small ubiquitin-like modifier (SUMO) recognition of a SUMO binding motif: a reversal of the bound orientation.. The Journal of biological chemistry, (2): [PMID:16204249] |
| 16. Wei, Huijun H and 6 more authors.. (2006) Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation.. The Journal of biological chemistry, (16): [PMID:16617055] |
| 17. Fu, Jihuan J, Xiong, Yanbao Y, Xu, Youli Y, Cheng, Genhong G and Tang, Hong H.. (2011) MDA5 is SUMOylated by PIAS2β in the upregulation of type I interferon signaling.. Molecular immunology, [PMID:21156324] |
| 18. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 19. Rabellino, Andrea A and 10 more authors.. (2012) The SUMO E3-ligase PIAS1 regulates the tumor suppressor PML and its oncogenic counterpart PML-RARA.. Cancer research, (1): [PMID:22406621] |
| 20. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364] |
| 21. Wang, Wen-Fang WF and 15 more authors.. (2017) HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells.. Nature communications, (25): [PMID:28842558] |
